斜纹夜蛾对茚虫威的抗性机制

被引：19

作者：

王建军 ^{[1
]}

董红刚 ^{[1
,2
]}

袁林泽 ^{[1
]}

机构：

[1] 扬州大学园艺与植物保护学院

[2] 扬州市邗江区植物保护站

来源：

植物保护学报 | 2009年 / 36卷 / 02期

关键词：

斜纹夜蛾; 茚虫威; 抗性机制; 酯酶; 钠离子通道;

D O I：

10.13802/j.cnki.zwbhxb.2009.02.012

中图分类号：

S433.4 [鳞翅目害虫];

学科分类号：

090401 [植物病理学];

摘要：

以斜纹夜蛾对茚虫威相对敏感种群和抗性倍数为15.63倍的抗性选育种群为材料,通过解毒酶活性测定与钠离子通道基因片段的克隆、测序,研究了斜纹夜蛾对茚虫威的抗性机制。结果表明,与相对敏感种群相比,抗性种群酯酶活性提高了2.27倍,但抗性和敏感种群谷胱甘肽S-转移酶和多功能氧化酶O-脱甲基活性无显著差异;3龄幼虫酯酶活性随着抗性指数的上升而逐渐提高;从相对敏感和抗性斜纹夜蛾种群3龄幼虫基因组DNA中扩增出位于钠离子通道IIS5-IIS6的341 bp DNA片段;与相对敏感种群相比,茚虫威抗性种群钠离子通道基因没有发生突变。

引用

页码：168 / 172

页数：5

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