碱性成纤维细胞生长因子抗局灶性脑缺血/再灌注引发神经细胞凋亡作用的实验研究

被引：5

作者：

杨琴

胡常林

机构：

[1] 重庆医科大学临床学院神经内科!重庆　

[2] 重庆医科大学第二临床学院神经内科!重庆　

来源：

重庆医科大学学报 | 2000年 / 04期

关键词：

缺血/再灌注; 凋亡; 碱性成纤维细胞生长因子;

D O I：

10.13406/j.cnki.cyxb.2000.04.004

中图分类号：

R743.31 [短暂性脑缺血];

学科分类号：

摘要：

目的　观察碱性成纤维细胞生长因子(bFGF)抗局灶性脑缺血/ 再灌注引发神经元凋亡作用。方法　采用线栓法制备大脑中动脉缺血/ 再灌注模型。术后首次即刻分别腹腔注射bFGF和生理盐水，以后每天一次，连用7天。TUNEL 法原位检测缺血后第1、2、3、5、7天脑石蜡切片含DNA 碎片的凋亡细胞。结果　正常组、假手术组、缺血组对侧半球每张切片有0～8个凋亡细胞，缺血/ 再灌注后缺血区凋亡细胞明显增多，缺血48h达高峰，以后逐渐降低，第7天仍可见凋亡细胞。bFGF组凋亡细胞数显著低于缺血/ 再灌注生理盐水对照组。结论　缺血/ 再灌注后缺血区神经元发生了凋亡，bFGF具有抗凋亡作用。

引用

页码：345 / 347

页数：3

共 11 条

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