糖基化终产物对小胶质细胞分泌IL-1β和TNF-α的影响

被引：10

作者：

王美霞

刘雪平

徐松

董传芳

侯亮

袁树华

机构：

[1] 山东大学附属省立医院老年神经科

来源：

山东大学学报(医学版) | 2011年 / 49卷 / 02期

关键词：

阿尔茨海默病; 糖基化终产物,高级; 白细胞介素1; 肿瘤坏死因子; 小神经胶质细胞; 大鼠,Wistar;

D O I：

暂无

中图分类号：

R749.16 [];

学科分类号：

摘要：

目的研究糖基化终产物(AGEs-BSA)对原代培养的小胶质细胞分泌白细胞介素1β(IL-1β)和肿瘤坏死因子α(TNF-α)的影响,在细胞水平探讨AGEs-BSA在阿尔茨海默病(AD)发生中的作用及其可能机制。方法体外培养小胶质细胞,用AGEs-BSA干预,用免疫细胞化学方法,进行鉴定并观察其形态变化;用AGEs-BSA300μg/mL激活和抗RAGE中和抗体阻断的方法对原代培养的小胶质细胞进行处理,用酶联免疫吸附法(ELISA)检测细胞上清液中IL-1β和TNF-α的水平。结果 AGEs-BSA干预后小胶质细胞胞体变大,形态不规则,呈"阿米巴样",细胞上清液中IL-1β、TNF-α浓度均明显升高(P<0.001),抗RAGE中和抗体+AGEs-BSA组细胞上清液IL-1β、TNF-α浓度较AGEs-BSA组呈下降趋势(P<0.01),但仍高于正常对照组(P<0.01)。结论 AGEs-BSA可激活小胶质细胞,诱导其释放IL-1β和TNF-α,并呈时间依赖性。提示糖基化终产物能直接或通过作用其受体激活小胶质细胞介导的免疫炎性反应。

引用

收藏

页码：34 / 38

页数：5

相关论文

共 8 条

[1]

RAGE, LRP-1, and amyloid-beta protein in Alzheimer's disease [J].

Donahue, John E. ;

Flaherty, Stephanie L. ;

Johanson, Conrad E. ;

Duncan, John A., III ;

Silverberg, Gerald D. ;

Miller, Miles C. ;

Tavares, Rosemarie ;

Yang, Wentian ;

Wu, Qian ;

Sabo, Edmond ;

Hovanesian, Virginia ;

Stopa, Edward G. .

ACTA NEUROPATHOLOGICA, 2006, 112 (04) :405-415

[2]

Bone marrow-derived microglia play a critical role in restricting senile plaque formation in Alzheimer's disease [J].

Simard, AR ;

Soulet, D ;

Gowing, G ;

Julien, JP ;

Rivest, S .

NEURON, 2006, 49 (04) :489-502

[3]

Exclusive induction of tau2 epitope in microglia/macrophages in inflammatory lesions - tautwopathy distinct from degenerative tauopathies [J].

Uchihara, T ;

Duyckaerts, C ;

Seilhean, D ;

Nakamura, A ;

Lazarini, F ;

Hauw, JJ .

ACTA NEUROPATHOLOGICA, 2005, 109 (02) :159-164

[4]

Interleukin-6 induces Alzheimer-type phosphorylation of tau protein by deregulating the cdk5/p35 pathway [J].

Quintanilla, RA ;

Orellana, DI ;

González-Billault, C ;

Maccioni, RB .

EXPERIMENTAL CELL RESEARCH, 2004, 295 (01) :245-257

[5]

Interleukin-1 promotion of MAPK-p38 overexpression in experimental animals and in Alzheimer's disease: potential significance for tau protein phosphorylation [J].

Sheng, JG ;

Jones, RA ;

Zhou, XQ ;

McGinness, JM ;

Van Eldik, LJ ;

Mrak, RE ;

Griffin, WST .

NEUROCHEMISTRY INTERNATIONAL, 2001, 39 (5-6) :341-348

[6]

AGES in brain ageing:: AGE-inhibitors as neuroprotective and anti-dementia drugs? [J].

Dukic-Stefanovic, S ;

Schinzel, R ;

Riederer, P ;

Münch, G .

BIOGERONTOLOGY, 2001, 2 (01) :19-34

[7]

Induction of CD40 on human endothelial cells by Alzheimer's β-amyloid peptides [J].

Tan, J ;

Town, T ;

Suo, Z ;

Wu, Y ;

Song, S ;

Kundtz, A ;

Kroeger, J ;

Humphrey, J ;

Crawford, F ;

Mullan, M .

BRAIN RESEARCH BULLETIN, 1999, 50 (02) :143-148

[8]

Advanced glycation end products in Alzheimer's disease and other neurodegenerative diseases [J].

Sasaki, N ;

Fukatsu, R ;

Tsuzuki, K ;

Hayashi, Y ;

Yoshida, T ;

Fujii, N ;

Koike, T ;

Wakayama, I ;

Yanagihara, R ;

Garruto, R ;

Amano, N ;

Makita, Z .

AMERICAN JOURNAL OF PATHOLOGY, 1998, 153 (04) :1149-1155