Geographic Differentiation of Essential Oil from Rhizome of Cultivated Atractylodes lancea by Using GC-MS and Chemical Pattern Recognition Analysis

被引：20

作者：

Song, Baohong ^{[1
]}

Wang, Wei ^{[1
]}

Liu, Ruipeng ^{[1
]}

Cai, Jinjin ^{[2
]}

Jiang, Yuanyuan ^{[1
]}

Tang, Xuemei ^{[3
]}

Wu, Hongfei ^{[1
]}

Ao, Hui ^{[1
,4
]}

Chen, Lu ^{[1
]}

机构：

[1] Chengdu Univ Tradit Chinese Med, Sch Pharm, State Key Lab Southwestern Chinese Med Resources, Chengdu 611137, Peoples R China

[2] Hosp Chengdu Univ Tradit Chinese Med, Chengdu 610075, Peoples R China

[3] Chengdu Inst Food Inspect, Chengdu 610045, Peoples R China

[4] Chengdu Univ Tradit Chinese Med, Innovat Inst Chinese Med & Pharm, Chengdu 611137, Peoples R China

来源：

MOLECULES | 2023年 / 28卷 / 05期

基金：

英国科研创新办公室;

关键词：

Atractylodes lancea; GC-MS; essential oil; chemical pattern recognition analysis; geographic origin; GAS-CHROMATOGRAPHY; FINGERPRINT; INHIBITION; APOPTOSIS; COMPONENT; HINESOL; GROWTH;

D O I：

10.3390/molecules28052216

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

The rhizome of Atractylodes lancea (RAL) is a well-known Chinese herbal medicine (CHM) that has been applied in clinical settings for thousands of years. In the past two decades, cultivated RAL has gradually replaced wild RAL and become mainstream in clinical practice. The quality of CHM is significantly influenced by its geographical origin. To date, limited studies have compared the composition of cultivated RAL from different geographical origins. As essential oil is the primary active component of RAL, a strategy combining gas chromatography-mass spectrometry (GC-MS) and chemical pattern recognition was first applied to compare the essential oil of RAL (RALO) from different regions in China. Total ion chromatography (TIC) revealed that RALO from different origins had a similar composition; however, the relative content of the main compounds varied significantly. In addition, 26 samples obtained from various regions were divided into three categories by hierarchical cluster analysis (HCA) and principal component analysis (PCA). Combined with the geographical location and chemical composition analysis, the producing regions of RAL were classified into three areas. The main compounds of RALO vary depending on the production areas. Furthermore, a one-way analysis of variance (ANOVA) revealed that there were significant differences in six compounds, including modephene, caryophyllene, gamma-elemene, atractylon, hinesol, and atractylodin, between the three areas. Hinesol, atractylon, and beta-eudesmol were selected as the potential markers for distinguishing different areas by orthogonal partial least squares discriminant analysis (OPLS-DA). In conclusion, by combining GC-MS with chemical pattern recognition analysis, this research has identified the chemical variations across various producing areas and developed an effective method for geographic origin tracking of cultivated RAL based on essential oils.

引用

页数：12

共 41 条

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