Hypoxia preconditioned DPSC-derived exosomes regulate angiogenesis via transferring LOXL2

被引：24

作者：

Li, Baoyu ^{[1
,2
,3
]}

Liang, Ailin ^{[1
,2
,3
]}

Zhou, Yanling ^{[1
,2
,3
]}

Huang, Yihua ^{[1
,2
,3
]}

Liao, Chenxi ^{[1
,2
,3
]}

Zhang, Xufang ^{[1
,2
,3
]}

Gong, Qimei ^{[1
,2
,3
]}

机构：

[1] Sun Yat Sen Univ, Hosp Stomatol, Guangzhou 510055, Peoples R China

[2] Guangdong Prov Key Lab Stomatol, Guangzhou 510080, Peoples R China

[3] Sun Yat Sen Univ, Guanghua Sch Stomatol, Guangzhou 510055, Peoples R China

来源：

EXPERIMENTAL CELL RESEARCH | 2023年 / 425卷 / 02期

关键词：

Hypoxia; Dental pulp stem cells; Exosomes; Angiogenesis; Proteomics; Lysyl oxidase-like 2; CROSS-LINKING; STEM-CELLS; LYSYL; NICHE;

D O I：

10.1016/j.yexcr.2023.113543

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 [肿瘤学];

摘要：

Hypoxia was proved to enhance the angiogenesis of stem cells. However, the mechanism of the angiogenic potential in hypoxia-pretreated dental pulp stem cells (DPSCs) is poorly understood. We previously confirmed that hypoxia enhances the angiogenic potential of DPSC-derived exosomes with upregulation of lysyl oxidase-like 2 (LOXL2). Therefore, our study aimed to illuminate whether these exosomes promote angiogenesis via transfer of LOXL2. Exosomes were generated from hypoxia-pretreated DPSCs (Hypo-Exos) stably silencing LOXL2 after lentiviral transfection and characterized with transmission electron microscopy, nanosight and Western blot. The efficiency of silencing was verified using quantitative real-time PCR (qRT-PCR) and Western blot. CCK-8, scratch and transwell assays were conducted to explore the effects of LOXL2 silencing on DPSCs proliferation and migration. Human umbilical vein endothelial cells (HUVECs) were co-incubated with exosomes to assess the migration and angiogenic capacity through transwell and matrigel tube formation assays. The relative expression of angiogenesis-associated genes was characterized by qRT-PCR and Western blot. LOXL2 was successfully silenced in DPSCs and inhibited DPSC proliferation and migration. LOXL2 silencing in Hypo-Exos partially reduced promotion of HUVEC migration and tube formation and inhibited the expression of angiogenesis-associated genes. Thus, LOXL2 is one of various factors mediating the angiogenic effects of Hypo-Exos.

引用

页数：10

共 45 条

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