New frontiers in gold labeling

被引：204

作者：

Hainfeld, JF ^{[1
]}

Powell, RD

机构：

[1] Brookhaven Natl Lab, Dept Biol, Upton, NY 11973 USA

[2] Nanoprobes Inc, Stony Brook, NY USA

来源：

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY | 2000年 / 48卷 / 04期

关键词：

gold; immunogold; electron microsocpy; gold labeling; gold clusters; immunocytochemistry; colloidal gold; Nanogold;

D O I：

10.1177/002215540004800404

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Recent advances in gold technology have led to probes with improved properties and performance for cell biologists: higher labeling density, better sensitivity, and greater penetration into tissues. Gold clusters, such as the 1.4-nm Nanogold, are gold compounds that can be covalently linked to Fab' antibody fragments, making small and stable probes. Silver enhancement then makes these small gold particles easily visible by EM, LM, and directly by eye. Another advance is the combination of fluorescent and gold probes for correlative microscopy. Chemical crosslinking of gold particles to many biologically active molecules has made possible many novel probes, such as gold-lipids, gold-Ni-NTA, and gold-ATP.

引用

页码：471 / 480

页数：10

共 60 条

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