Ultrasound-mediated transfection of canine myocardium by intravenous administration of cationic microbubble-linked plasmid DNA

被引：96

作者：

Vannan, M

McCreery, T

Li, P

Han, ZG

Unger, E

Kuersten, B

Nabel, E

Rajagopalan, S

机构：

[1] Univ Michigan, Dept Internal Med, Div Cardiol, Hlth Syst, Ann Arbor, MI 48109 USA

[2] Univ Arizona, Dept Radiol, Tucson, AZ 85724 USA

[3] ImaRx Therapeut Inc, Tucson, AZ USA

[4] NIH, Bethesda, MD 20892 USA

来源：

JOURNAL OF THE AMERICAN SOCIETY OF ECHOCARDIOGRAPHY | 2002年 / 15卷 / 03期

关键词：

D O I：

10.1067/mje.2002.119913

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

We tested the hypothesis that targeted disruption of cationic microbubble-linked plasmid DNA, using diagnostic ultrasound, may aid transfection of large animal myocardium. Plasinid DNA encoding for CAT (pCAT, chloramphenicol acetyltransferase) was bound to a novel cationic microbubble containing MRX-225 for intravenous administration, and 16 dogs in 4 groups variously received this conjugate or plasmid only, or were exposed to ultrasound. Histochemical staining and enzyme-linked immuno-sorbent assay analysis showed CAT activity in the myocardium of only those animals that received microbubble-linked DNA and were exposed to ultrasound. Thus, disruption of cationic-linked, low-dose plasmid systems by diagnostic ultrasound may facilitate transfection of large animal hearts.

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收藏

页码：214 / 218

页数：5

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