Phytochelatin synthesis is not responsible for Cd tolerance in the Zn/Cd hyperaccumulator Thlaspi caerulescenes (J. and C.!Presl)

被引:155
作者
Ebbs, S [1 ]
Lau, I
Ahner, B
Kochian, L
机构
[1] So Illinois Univ, Dept Plant Biol, Carbondale, IL 62901 USA
[2] Cornell Univ, Dept Agr & Biol Engn, Ithaca, NY 14853 USA
[3] Cornell Univ, USDA ARS, US Plant Soil & Nutr Lab, Ithaca, NY 14853 USA
关键词
cadmium; hyperaccumulator; metal tolerance; phytochelatin; Thlaspi (Cd tolerance);
D O I
10.1007/s004250100650
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Thlaspi caerulescens (J. & C. Presl, "Prayon") is a heavy-metal hyperaccumulator that accumulates Zn and Cd to high concentrations (40,000 and 4,000 mg kg DW-1, respectively) without phytotoxicity. The mechanism of Cd tolerance has not been characterized but reportedly involves vacuolar sequestration. The role of phytochelatins (PCs) in metal tolerance in T. caerulescens and the related non-accumulator T. arvense was examined. Although PCs were produced by both species in response to Cd, these peptides do not appear to be involved in metal tolerance in the hyperaccumulator. Leaf and root PC levels for both species showed a similar positive correlation with tissue Cd, but total PC levels in the hyperaccumulator were generally lower, despite correspondingly higher metal concentrations. The lack of a role for PCs in the hyperaccumulator's response to metal stress suggests that other mechanisms are responsible Cd tolerance. The lower level of leaf PCs in T. caerulescens also implies that Cd in the shoot is sequestered in a compartment or form that does not elicit a PC response.
引用
收藏
页码:635 / 640
页数:6
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