The essential yeast proteins Yra1 and Sub2 are messenger RNA export factors that have conserved counterparts in metazoans, designated Aly and UAP56, respectively(1-7). These factors couple the machineries that function in splicing and export of mRNA(1-7). Here we show that both Yra1 and Sub2 are stoichiometrically associated with the heterotetrameric THO complex(8),which functions in transcription in yeast(8-11). We also show that Sub2 and Yra1 interact genetically with all four components of the THO complex (Tho2, Hpr1, Mft1 and Thp2). Moreover, these components operate in the export of bulk poly(A)(+) RNA as well as of mRNA derived from intronless genes. Both Aly and UAP56 associate with human counterparts of the THO complex. Together, these data define a conserved complex, designated the TREX ('transcription/export') complex. The TREX complex is specifically recruited to activated genes during transcription and travels the entire length of the gene with RNA polymerase II. Our data indicate that the TREX complex has a conserved role in coupling transcription to mRNA export.
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UNIV MASSACHUSETTS, MED CTR, PROGRAM MOL MED, HOWARD HUGHES MED INST, WORCESTER, MA 01605 USAUNIV MASSACHUSETTS, MED CTR, PROGRAM MOL MED, HOWARD HUGHES MED INST, WORCESTER, MA 01605 USA
Fleckner, J
Zhang, M
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UNIV MASSACHUSETTS, MED CTR, PROGRAM MOL MED, HOWARD HUGHES MED INST, WORCESTER, MA 01605 USAUNIV MASSACHUSETTS, MED CTR, PROGRAM MOL MED, HOWARD HUGHES MED INST, WORCESTER, MA 01605 USA
Zhang, M
Valcarcel, J
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UNIV MASSACHUSETTS, MED CTR, PROGRAM MOL MED, HOWARD HUGHES MED INST, WORCESTER, MA 01605 USAUNIV MASSACHUSETTS, MED CTR, PROGRAM MOL MED, HOWARD HUGHES MED INST, WORCESTER, MA 01605 USA
Valcarcel, J
Green, MR
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UNIV MASSACHUSETTS, MED CTR, PROGRAM MOL MED, HOWARD HUGHES MED INST, WORCESTER, MA 01605 USAUNIV MASSACHUSETTS, MED CTR, PROGRAM MOL MED, HOWARD HUGHES MED INST, WORCESTER, MA 01605 USA
机构:
Univ Calif San Francisco, Dept Biochem & Biophys, San Francisco, CA 94143 USAUniv Calif San Francisco, Dept Biochem & Biophys, San Francisco, CA 94143 USA
Kistler, AL
Guthrie, C
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Univ Calif San Francisco, Dept Biochem & Biophys, San Francisco, CA 94143 USAUniv Calif San Francisco, Dept Biochem & Biophys, San Francisco, CA 94143 USA
机构:
UNIV MASSACHUSETTS, MED CTR, PROGRAM MOL MED, HOWARD HUGHES MED INST, WORCESTER, MA 01605 USAUNIV MASSACHUSETTS, MED CTR, PROGRAM MOL MED, HOWARD HUGHES MED INST, WORCESTER, MA 01605 USA
Fleckner, J
Zhang, M
论文数: 0引用数: 0
h-index: 0
机构:
UNIV MASSACHUSETTS, MED CTR, PROGRAM MOL MED, HOWARD HUGHES MED INST, WORCESTER, MA 01605 USAUNIV MASSACHUSETTS, MED CTR, PROGRAM MOL MED, HOWARD HUGHES MED INST, WORCESTER, MA 01605 USA
Zhang, M
Valcarcel, J
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h-index: 0
机构:
UNIV MASSACHUSETTS, MED CTR, PROGRAM MOL MED, HOWARD HUGHES MED INST, WORCESTER, MA 01605 USAUNIV MASSACHUSETTS, MED CTR, PROGRAM MOL MED, HOWARD HUGHES MED INST, WORCESTER, MA 01605 USA
Valcarcel, J
Green, MR
论文数: 0引用数: 0
h-index: 0
机构:
UNIV MASSACHUSETTS, MED CTR, PROGRAM MOL MED, HOWARD HUGHES MED INST, WORCESTER, MA 01605 USAUNIV MASSACHUSETTS, MED CTR, PROGRAM MOL MED, HOWARD HUGHES MED INST, WORCESTER, MA 01605 USA
机构:
Univ Calif San Francisco, Dept Biochem & Biophys, San Francisco, CA 94143 USAUniv Calif San Francisco, Dept Biochem & Biophys, San Francisco, CA 94143 USA
Kistler, AL
Guthrie, C
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif San Francisco, Dept Biochem & Biophys, San Francisco, CA 94143 USAUniv Calif San Francisco, Dept Biochem & Biophys, San Francisco, CA 94143 USA