TREX is a conserved complex coupling transcription with messenger RNA export

被引:684
作者
Strässer, K
Masuda, S
Mason, P
Pfannstiel, J
Oppizzi, M
Rodriguez-Navarro, S
Rondón, AG
Aguilera, A
Struhl, K
Reed, R [1 ]
Hurt, E
机构
[1] Harvard Univ, Sch Med, Dept Cell Biol, Boston, MA 02115 USA
[2] Heidelberg Univ, BZH, D-69120 Heidelberg, Germany
[3] Harvard Univ, Sch Med, Dept Biol Chem & Mol Pharmacol, Boston, MA 02115 USA
[4] Univ Seville, Dept Genet, E-41012 Seville, Spain
基金
美国国家卫生研究院;
关键词
D O I
10.1038/nature746
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The essential yeast proteins Yra1 and Sub2 are messenger RNA export factors that have conserved counterparts in metazoans, designated Aly and UAP56, respectively(1-7). These factors couple the machineries that function in splicing and export of mRNA(1-7). Here we show that both Yra1 and Sub2 are stoichiometrically associated with the heterotetrameric THO complex(8),which functions in transcription in yeast(8-11). We also show that Sub2 and Yra1 interact genetically with all four components of the THO complex (Tho2, Hpr1, Mft1 and Thp2). Moreover, these components operate in the export of bulk poly(A)(+) RNA as well as of mRNA derived from intronless genes. Both Aly and UAP56 associate with human counterparts of the THO complex. Together, these data define a conserved complex, designated the TREX ('transcription/export') complex. The TREX complex is specifically recruited to activated genes during transcription and travels the entire length of the gene with RNA polymerase II. Our data indicate that the TREX complex has a conserved role in coupling transcription to mRNA export.
引用
收藏
页码:304 / 308
页数:5
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