Neural toxicity of retroviruses

被引：6

作者：

Hurley, MJ

Bradford, HF

OHare, K

Stern, GM

Uchida, K

机构：

[1] UCL, SCH MED, DEPT CLIN NEUROL, LONDON W1N 8AA, ENGLAND

[2] KEIO UNIV, SCH MED, DEPT NEUROSURG, TOKYO 160, JAPAN

来源：

NEUROSCIENCE LETTERS | 1996年 / 220卷 / 01期

关键词：

cell culture; tyrosine hydroxylase; retrovirus; ventral mesencephalon; cerebral cortex; rat;

D O I：

10.1016/S0304-3940(96)13224-9

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Recombinant retroviruses containing the cDNA for human tyrosine hydroxylase-l and Escherichia coli lacZ gene were used to infect primary foetal ventral mesencephalon and cortical cultures from rat brain. Severe neuronal toxicity resulted 3-4 days after infection, glial cells seemed to be much more resistant. The toxicity was likely to have resulted from an agent present within the virus-containing medium itself, rather than from the retrovirus itself. The results of this study indicate that retroviruses are not suitable vectors for the introduction of tyrosine hydroxylase into primary neuronal cultures. Copyright (C) 1996 Elsevier Science Ireland Ltd.

引用

收藏

页码：66 / 68

页数：3

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