Protection against heat stress-induced oxidative damage in arabidopsis involves calcium, abscisic acid, ethylene, and salicylic acid

被引:652
作者
Larkindale, J [1 ]
Knight, MR [1 ]
机构
[1] Univ Oxford, Dept Plant Sci, Oxford OX1 3RB, England
关键词
D O I
10.1104/pp.010320
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Plants, in common with all organisms, have evolved mechanisms to cope with the problems caused by high temperatures. We examined specifically the involvement of calcium, abscisic acid (ABA), ethylene, and salicylic acid (SA) in the protection against heat-induced oxidative damage in Arabidopsis. Heat caused increased thiobarbituric acid reactive substance levels (an indicator of oxidative damage to membranes) and reduced survival. Both effects required light and were reduced in plants that had acquired thermotolerance through a mild heat pretreatment. Calcium channel blockers and calmodulin inhibitors increased these effects of heating and added calcium reversed them, implying that protection against heat-induced oxidative damage in Arabidopsis requires calcium and calmodulin. Similar to calcium, SA, 1-aminocyclopropane-1-carboxylic acid (a precursor to ethylene), and ABA added to plants protected them from heat-induced oxidative damage. In addition, the ethylene-insensitive mutant etr-1, the ABA-insensitive mutant abi-1, and a transgenic line expressing nahG (consequently inhibited in SA production) showed increased susceptibility to heat. These data suggest that protection against heat-induced oxidative damage in Arabidopsis also involves ethylene, ABA, and SA. Real time measurements of cytosolic calcium levels during heating in Arabidopsis detected no increases in response to heat per se, but showed transient elevations in response to recovery from heating. The magnitude of these calcium peaks was greater in thermotolerant plants, implying that these calcium signals might play a role in mediating the effects of acquired thermotolerance. Calcium channel blockers and calmodulin inhibitors added solely during the recovery phase suggest that this role for calcium is in protecting against oxidative damage specifically during/after recovery.
引用
收藏
页码:682 / 695
页数:14
相关论文
共 52 条
[1]   Identification and characterization of a heat-stress induced gene in cabbage encodes a Kunitz type protease inhibitor [J].
Annamalai, P ;
Yanagihara, S .
JOURNAL OF PLANT PHYSIOLOGY, 1999, 155 (02) :226-233
[2]   MULTIPLE INDUCERS OF THE DROSOPHILA HEAT-SHOCK LOCUS-93D (HSR-OMEGA) - INDUCER-SPECIFIC PATTERNS OF THE 3 TRANSCRIPTS [J].
BENDENA, WG ;
GARBE, JC ;
TRAVERSE, KL ;
LAKHOTIA, SC ;
PARDUE, ML .
JOURNAL OF CELL BIOLOGY, 1989, 108 (06) :2017-2028
[4]   Identification of genetic diversity and mutations in higher plant acquired thermotolerance [J].
Burke, JJ .
PHYSIOLOGIA PLANTARUM, 2001, 112 (02) :167-170
[5]   Calcium imaging shows differential sensitivity to cooling and communication in luminous transgenic plants [J].
Campbell, AK ;
Trewavas, AJ ;
Knight, MR .
CELL CALCIUM, 1996, 19 (03) :211-218
[6]   Changes in salicylic acid and antioxidants during induced thermotolerance in mustard seedlings [J].
Dat, JF ;
Foyer, CH ;
Scott, IM .
PLANT PHYSIOLOGY, 1998, 118 (04) :1455-1461
[7]   A CENTRAL ROLE OF SALICYLIC-ACID IN PLANT-DISEASE RESISTANCE [J].
DELANEY, TP ;
UKNES, S ;
VERNOOIJ, B ;
FRIEDRICH, L ;
WEYMANN, K ;
NEGROTTO, D ;
GAFFNEY, T ;
GUTRELLA, M ;
KESSMANN, H ;
WARD, E ;
RYALS, J .
SCIENCE, 1994, 266 (5188) :1247-1250
[8]   Hydrogen peroxide- and glutathione-associated mechanisms of acclimatory stress tolerance and signalling [J].
Foyer, CH ;
LopezDelgado, H ;
Dat, JF ;
Scott, IM .
PHYSIOLOGIA PLANTARUM, 1997, 100 (02) :241-254
[9]   PROTECTION AGAINST OXYGEN RADICALS - AN IMPORTANT DEFENSE-MECHANISM STUDIED IN TRANSGENIC PLANTS [J].
FOYER, CH ;
DESCOURVIERES, P ;
KUNERT, KJ .
PLANT CELL AND ENVIRONMENT, 1994, 17 (05) :507-523
[10]   Heat-shock-induced changes in intracellular Ca2+ level in tobacco seedlings in relation to thermotolerance [J].
Gong, M ;
van der Luit, AH ;
Knight, MR ;
Trewavas, AJ .
PLANT PHYSIOLOGY, 1998, 116 (01) :429-437