Indomethacin Inhibits Cancer Cell Migration via Attenuation of Cellular Calcium Mobilization

被引：57

作者：

Guo, Yuh-Cherng ^{[1
]}

Chang, Che-Mai ^{[2
,3
]}

Hsu, Wen-Li ^{[4
]}

Chiu, Siou-Jin ^{[5
]}

Tsai, Yao-Ting ^{[2
]}

Chou, Yii-Her ^{[5
]}

Hou, Ming-Feng ^{[6
,7
,8
]}

Wang, Jaw-Yan ^{[6
,7
,8
]}

Lee, Mei-Hsien ^{[9
]}

Tsai, Ke-Li ^{[4
]}

Chang, Wei-Chiao ^{[2
,3
,6
,10
]}

机构：

[1] Changhua Christian Hosp, Dept Neurol, Changhua 500, Taiwan

[2] Taipei Med Univ, Sch Pharm, Dept Clin Pharm, Taipei 11031, Taiwan

[3] Taipei Med Univ, Sch Pharm, Master Program Clin Pharmacogenom & Pharmacoprote, Taipei 11031, Taiwan

[4] Kaohsiung Med Univ, Coll Med, Dept Physiol, Kaohsiung 807, Taiwan

[5] Kaohsiung Med Univ, Kaohsiung Med Univ Hosp, Coll Med, Dept Urol, Kaohsiung 807, Taiwan

[6] Kaohsiung Med Univ, Kaohsiung Med Univ Hosp, Ctr Canc, Kaohsiung 807, Taiwan

[7] Kaohsiung Med Univ, Kaohsiung Med Univ Hosp, Dept Surg, Div Gastroenterol & Gen Surg, Kaohsiung 807, Taiwan

[8] Kaohsiung Med Univ, Coll Med, Grad Inst Clin Med, Kaohsiung 807, Taiwan

[9] Taipei Med Univ, Coll Pharm, Grad Inst Pharmacognosy, Taipei 11031, Taiwan

[10] Taipei Med Univ, Wanfang Hosp, Dept Pharm, Taipei 11031, Taiwan

来源：

MOLECULES | 2013年 / 18卷 / 06期

关键词：

indomethacin; EGF; COX-2; cell migration; EPIDERMAL-GROWTH-FACTOR; FACTOR RECEPTORS; GENE ACTIVATION; BINDING-SITES; LUNG-CANCER; CYCLOOXYGENASE-2; PROLIFERATION; DRUGS; VINCULIN; TARGETS;

D O I：

10.3390/molecules18066584

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Non-steroidal anti-inflammatory drugs (NSAIDs) were shown to reduce the risk of colorectal cancer recurrence and are widely used to modulate inflammatory responses. Indomethacin is an NSAID. Herein, we reported that indomethacin can suppress cancer cell migration through its influence on the focal complexes formation. Furthermore, endothelial growth factor (EGF)-mediated Ca2+ influx was attenuated by indomethacin in a dose dependent manner. Our results identified a new mechanism of action for indomethacin: inhibition of calcium influx that is a key determinant of cancer cell migration.

引用

页码：6584 / 6596

页数：13

共 29 条

[1]

HIERARCHY OF BINDING-SITES FOR GRB2 AND SHC ON THE EPIDERMAL GROWTH-FACTOR RECEPTOR [J].

BATZER, AG ;

ROTIN, D ;

URENA, JM ;

SKOLNIK, EY ;

SCHLESSINGER, J .

MOLECULAR AND CELLULAR BIOLOGY, 1994, 14 (08) :5192-5201

[2]

EVIDENCE THAT INDOMETHACIN REVERSIBLY INHIBITS CELL-GROWTH IN THE G1 PHASE OF THE CELL-CYCLE [J].

BAYER, BM ;

BEAVEN, MA .

BIOCHEMICAL PHARMACOLOGY, 1979, 28 (03) :441-443

[3]

Stimulation of cytosolic and mitochondrial calcium mobilization by indomethacin in Caco-2 cells: Modulation by the polyphenols quercetin, resveratrol and rutin [J].

Carrasco-Pozo, Catalina ;

Pastene, Edgar ;

Vergara, Carola ;

Zapata, Moises ;

Sandoval, Cristian ;

Gotteland, Martin .

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 2012, 1820 (12) :2052-2061

[4]

Ca2+ influx through CRAC channels activates cytosolic phospholipase A2, leukotriene C4 secretion, and expression of c-fos through ERK-dependent and -independent pathways in mast cells [J].

Chang, Wei-Chiao ;

Nelson, Charmaine ;

Parekh, Anant B. .

FASEB JOURNAL, 2006, 20 (13) :2381-+

[5]

Cyclooxygenase enzymes as targets for therapeutic intervention in inflammation [J].

Colville-Nash, PR ;

Gilroy, DW .

DRUG NEWS & PERSPECTIVES, 2000, 13 (10) :587-597

[6]

Comparative effects of indomethacin on cell proliferation and cell cycle progression in tumor cells grown in vitro and in vivo [J].

Eli, Y ;

Przedecki, F ;

Levin, G ;

Kariv, N ;

Raz, A .

BIOCHEMICAL PHARMACOLOGY, 2001, 61 (05) :565-571

[7]

EGF enhances the migration of cancer cells by up-regulation of TRPM7 [J].

Gao, Haixia ;

Chen, Xingjuan ;

Du, Xiaona ;

Guan, Bingcai ;

Liu, Yani ;

Zhang, Hailin .

CELL CALCIUM, 2011, 50 (06) :559-568

[8]

Vinculin, cell mechanics and tumour cell invasion [J].

Goldmann, Wolfgang H. ;

Auernheimer, Vera ;

Thievessen, Ingo ;

Fabry, Ben .

CELL BIOLOGY INTERNATIONAL, 2013, 37 (05) :397-405

[9]

Harris Randall E, 2007, Subcell Biochem, V42, P93

[10]

Harris, Randall E. ;

Beebe-Donk, Joanne ;

Alshafie, Galal A. .

BMC CANCER, 2008, 8 (1)

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