Twist-1 regulates the miR-199a/214 cluster during development

被引：200

作者：

Lee, Youn-Bok ^{[1
]}

Bantounas, Ioannis ^{[1
]}

Lee, Do-Young ^{[1
]}

Phylactou, Leonidas ^{[2
]}

Caldwell, Maeve A. ^{[1
]}

Uney, James B. ^{[1
]}

机构：

[1] Univ Bristol, Henry Wellcome Labs Integrated Neurosci & Endocri, Bristol BS1 3NY, Avon, England

[2] Cyprus Inst Neurol & Genet, CY-1683 Nicosia, Cyprus

来源：

NUCLEIC ACIDS RESEARCH | 2009年 / 37卷 / 01期

基金：

英国惠康基金;

关键词：

HUMAN OVARIAN-CANCER; TRANSCRIPTION FACTORS; EXPRESSION; MOUSE; PROTEIN; CELLS; GENE; METASTASIS; DROSOPHILA; MICRORNAS;

D O I：

10.1093/nar/gkn920

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

MicroRNAs are known to regulate developmental processes but their mechanism of regulation remains largely uncharacterized. We show the transcription factor Twist-1 drives the expression of a 7.9-kb noncoding RNA transcript (from the Dynamin-3 gene intron) that encodes a miR-199a and miR-214 cluster. We also show that knocking down Twist-1 with shRNAs decreased miR-199a/214 levels and that Twist-1 bound an E-Box promoter motif to developmentally regulate the expression of these miRNAs. The expression of HIF-1 (known to mediate Twist-1 transcription), miR-199a and miR-214 was maximal at E12.5 and the miRNAs were expressed specifically in mouse cerebellum, midbrain, nasal process and fore- and hindlimb buds. This study shows the expression of the miR199a/214 cluster is controlled by Twist-1 via an E-Box promoter element and supports a role for these miRNAs as novel intermediates in the pathways controlling the development of specific neural cell populations.

引用

页码：123 / 128

页数：6

共 28 条

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Relief of microRNA-mediated translational repression in human cells subjected to stress [J].