Poly(vinyl chloride), polysulfone and sulfonated polyether-ether sulfone composite membranes for glucose and hydrogen peroxide perm-selectivity in amperometric biosensors

被引:26
作者
Benmakroha, Y [1 ]
Christie, I [1 ]
Desai, M [1 ]
Vadgama, P [1 ]
机构
[1] UNIV MANCHESTER,HOPE HOSP,DEPT CLIN BIOCHEM,SALFORD M6 8HD,LANCS,ENGLAND
关键词
membrane; polysulfone; sulfonated polyether-ether sulphone-polyether sulfone; biosensor; glucose; amperometry; poly(vinyl chloride);
D O I
10.1039/an9962100521
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Membrane materials were evaluated for use in amperometric biosensors, PVC, polysulfone (PS) and sulfonated polyether-ether sulfone-polyether sulfone (SPEES-PES) and SPEES-PES/PVC composites were solvent cast, Varying the plasticizer (isopropyl myristate) content of PS and PVC membranes enabled electrode response to hydrogen peroxide and acetaminophen to be modulated, Omission of plasticizer from PS and PVC resulted in hydrogen peroxide permeable membranes, selective against acetaminophen, ascorbate and urate (ratios greater than or equal to 230:1 in each case), SPEES-PES copolymer membranes (unplasticized) showed greater hydrogen peroxide permeability (corresponding ratios greater than or equal to 1430:1), By mixing plasticized PVC and SPEES-PES in suitable proportions, permeability ratios of hydrogen peroxide: acetaminophen were controlled, Glucose sensors using these composite membranes for H2O2 perm-selectivity demonstrated linear responses through the clinical range, and less than or equal to 5% loss of signal on exposure to blood, A novel form of glucose oxidase based biosensor deployed a new membrane as an outer layer selective for the glucose substrate, rejecting interferents. Accordingly, a second-selective layer for the enzyme reaction product (hydrogen peroxide) is not required, The new membrane is a plasticized mix of PVC and SPEES-PES polymers incorporating isopropyl myristate, Selectivity is adequate for clinical glucose measurement, and in particular acetaminophen interference is excluded in addition to ascorbate and urate, representing an advance over membranes described hitherto, A linear range of 0-20 mmol l(-1) glucose confirms control of substrate diffusion sufficient to extend apparent enzyme K-M, and resistance to fouling in blood is also promising for deployment in a clinical assay.
引用
收藏
页码:521 / 526
页数:6
相关论文
共 22 条
[1]   SULFONATION OF POLY(PHENYLENE ETHER SULPHONE)S CONTAINING HYDROQUINONE RESIDUES [J].
BUNN, A ;
ROSE, JB .
POLYMER, 1993, 34 (05) :1114-1116
[2]  
BUTTRY DA, 1983, J AM CHEM SOC, V105, P684
[3]   FERROCENE-MEDIATED ENZYME ELECTRODE FOR AMPEROMETRIC DETERMINATION OF GLUCOSE [J].
CASS, AEG ;
DAVIS, G ;
FRANCIS, GD ;
HILL, HAO ;
ASTON, WJ ;
HIGGINS, IJ ;
PLOTKIN, EV ;
SCOTT, LDL ;
TURNER, APF .
ANALYTICAL CHEMISTRY, 1984, 56 (04) :667-671
[4]   MODIFICATION OF ELECTRODE SURFACES WITH OXIDIZED PHENOLS TO CONFER SELECTIVITY TO AMPEROMETRIC BIOSENSORS FOR GLUCOSE DETERMINATION [J].
CHRISTIE, IM ;
VADGAMA, P ;
LLOYD, S .
ANALYTICA CHIMICA ACTA, 1993, 274 (02) :191-199
[5]   PLASTICIZED POLY(VINYL CHLORIDE) AS A PERMSELECTIVE BARRIER MEMBRANE FOR HIGH-SELECTIVITY AMPEROMETRIC SENSORS AND BIOSENSORS [J].
CHRISTIE, IM ;
TRELOAR, PH ;
VADGAMA, P .
ANALYTICA CHIMICA ACTA, 1992, 269 (01) :65-73
[6]  
CHUA KS, 1978, CLIN CHEM, V24, P150
[7]   SENSORS FROM POLYMER MODIFIED ELECTRODES [J].
ESPENSCHEID, MW ;
GHATAKROY, AR ;
MOORE, RB ;
PENNER, RM ;
SZENTIRMAY, MN ;
MARTIN, CR .
JOURNAL OF THE CHEMICAL SOCIETY-FARADAY TRANSACTIONS I, 1986, 82 :1051-1070
[8]   SELECTIVE DETECTION IN FLOW-ANALYSIS BASED ON THE COMBINATION OF IMMOBILIZED ENZYMES AND CHEMICALLY MODIFIED ELECTRODES [J].
GORTON, L ;
CSOREGI, E ;
DOMINGUEZ, E ;
EMNEUS, J ;
JONSSONPETTERSSON, G ;
MARKOVARGA, G ;
PERSSON, B .
ANALYTICA CHIMICA ACTA, 1991, 250 (01) :203-248
[9]  
HIGSON SPJ, 1993, ANAL CHIM ACTA, V276, P335, DOI 10.1016/0003-2670(93)80401-6
[10]   AMPEROMETRIC ENZYME ELECTRODE BIOFOULING AND PASSIVATION IN BLOOD - CHARACTERIZATION OF WORKING ELECTRODE POLARIZATION AND INNER MEMBRANE EFFECTS [J].
HIGSON, SPJ ;
DESAI, MA ;
GHOSH, S ;
CHRISTIE, I ;
VADGAMA, P .
JOURNAL OF THE CHEMICAL SOCIETY-FARADAY TRANSACTIONS, 1993, 89 (15) :2847-2851