Near-field scanning optical microscopy detects nanoscale glycolipid domains in the plasma membrane

被引：9

作者：

Abulrob, A.

Lu, Z. ^{[1
]}

Brunette, E.

Pulla, D. ^{[1
]}

Stanimirovic, D.

Johnston, L. J. ^{[1
]}

机构：

[1] Natl Res Council Canada, Steacie Inst Mol Sci, Ottawa, ON K1A 0R6, Canada

来源：

JOURNAL OF MICROSCOPY | 2008年 / 232卷 / 02期

关键词：

Confocal microscopy; glycolipid; membrane rafts; near-field scanning optical microscopy;

D O I：

10.1111/j.1365-2818.2008.02093.x

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

The localization of asialo-GM1 in ordered membrane raft domains in HeLa cells has been examined using a combination of membrane fractionation and fluorescence imaging. The glycolipid is enriched in Triton X-100 insoluble membrane fractions that contain high concentrations of cholesterol and caveolin-1 but is also found in detergent soluble membrane fractions. Near-field fluorescence microscopy shows that a fraction of the asialo-GM1 is localized in small nanoscale clusters that have an upper limit for the average diameter of approximately 90 nm and are partially colocalized with caveolae membrane domains. In addition to clusters, a diffuse, non-clustered population of asialo-GM1 is observed and is hypothesized to correspond to glycolipid isolated in detergent soluble membrane fractions.

引用

页码：225 / 234

页数：10

共 36 条

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