Evaluation of immunity and protective efficacy of a dengue-3 premembrane and envelope DNA vaccine in Aotus nancymae monkeys

被引：36

作者：

Blair, PJ ^{[1
]}

Kochel, TJ ^{[1
]}

Raviprakash, K ^{[1
]}

Guevara, C ^{[1
]}

Salazar, M ^{[1
]}

Wu, SJ ^{[1
]}

Olson, JG ^{[1
]}

Porter, KR ^{[1
]}

机构：

[1] Naval Med Res Ctr, Silver Spring, MD 20910 USA

来源：

VACCINE | 2006年 / 24卷 / 09期

关键词：

dengue; DNA vaccine; Aotus monkeys;

D O I：

10.1016/j.vaccine.2005.09.032

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A dengue (DEN) virus type 3 DNA vaccine expressing pre-membrane and envelope genes was tested for immunogenicity and protective efficacy in Aotus monkeys. Five of six vaccinated animals demonstrated moderate DEN-specific antibody responses as measured by ELISA and virus neutralization in vitro. By contrast, none of the six control animals developed detectable anti-DEN antibodies. When five vaccinated animals were challenged with live DEN-3 virus and viremia determined by PCR amplification of viral RNA in serum samples, one animal was completely protected and two were partially protected as indicated by a decrease in mean days of viremia. The results demonstrate the ability of the DEN-3 DNA vaccine to elicit a neutralizing antibody response and to partially protect against live virus challenge. These findings support the inclusion of this construct in a tetravalent DNA vaccine. Published by Elsevier Ltd.

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收藏

页码：1427 / 1432

页数：6

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