Experimental colitis attenuates development of toxin-induced cholangitis in rats

被引：42

作者：

Tjandra, K ^{[1
]}

Le, T ^{[1
]}

Swain, MG ^{[1
]}

机构：

[1] Univ Calgary, Hlth Sci Ctr, Gastrointestinal Res Grp, Liver Unit, Calgary, AB T2N 4N1, Canada

来源：

DIGESTIVE DISEASES AND SCIENCES | 2002年 / 47卷 / 06期

关键词：

experimental colitis; cholangitis; alpha-naphthylisothiocyanate; 2,4,6-trinitrobenzene sulfonic acid; interleukin-10;

D O I：

10.1023/A:1015330809095

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

Primary sclerosing cholangitis frequently occurs in association with ulcerative colitis. This close association may be due to colitis predisposing patients to bile ductular injury. Therefore, we determined the susceptibility of rats with experimental colitis to toxin-induced cholangitis. Sprague-Dawley rats received 2,4,6-trinitrobenzene-sulfonic-acid (TNBS) or ethanol vehicle intracolonically. Seven days later, rats received either the biliary epithelial cell toxin alpha-naphthylisothiocyanate (ANIT) or vehicle and were killed 24 hr later. Liver histology, serum biochemistries and tumor-necrosis factor-alpha (TNF-alpha), and hepatic interleukin-10 (IL-10) mRNA were determined. TNBS-treated rats showed extensive macroscopic colonic damage and a 10-fold increase in myeloperoxidase activity compared to ethanol-treated controls. ANIT-treated noncolitic rats showed portal inflammation centered on damaged bile ducts (cholangitis), which was markedly attenuated in ANIT-treated colitic rats. Hepatic IL-10 mRNA was twofold higher in colitic compared to noncolitic rats, with no difference in serum TNF-alpha. In conclusion, experimental colitis attenuates the development of toxin-induced cholangitis in rats, possibly by up-regulating hepatic IL-10 expression.

引用

页码：1216 / 1223

页数：8

共 43 条

[1]

MEASUREMENT OF CUTANEOUS INFLAMMATION - ESTIMATION OF NEUTROPHIL CONTENT WITH AN ENZYME MARKER [J].

BRADLEY, PP ;

PRIEBAT, DA ;

CHRISTENSEN, RD ;

ROTHSTEIN, G .

JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1982, 78 (03) :206-209

[2]

EXPERIMENTAL COLITIS IN RATS INDUCES LOW-GRADE ENDOTOXINEMIA WITHOUT HEPATOBILIARY ABNORMALITIES [J].

BRAND, HS ;

MAAS, MAW ;

BOSMA, A ;

VANKETEL, RJ ;

SPEELMAN, P ;

CHAMULEAU, RAFM .

DIGESTIVE DISEASES AND SCIENCES, 1994, 39 (06) :1210-1215

[3]

ENDOTOXIN TOLERANCE AND POLYMYXIN-B MODIFY LIVER-DAMAGE AND CHOLESTASIS INDUCED BY A SINGLE DOSE OF ALPHA-NAPHTHYLISOTHIOCYANATE IN THE RAT [J].

CALCAMUGGI, G ;

LANZIO, M ;

DUGHERA, L ;

BABINI, G ;

EMANUELLI, G .

ARCHIVES OF TOXICOLOGY, 1992, 66 (02) :126-130

[4]

CANGEMI JR, 1989, GASTROENTEROLOGY, V96, P790

[5]

PRIMARY SCLEROSING CHOLANGITIS - A REVIEW OF ITS CLINICAL-FEATURES, CHOLANGIOGRAPHY, AND HEPATIC HISTOLOGY [J].

CHAPMAN, RWG ;

ARBORGH, BAM ;

RHODES, JM ;

SUMMERFIELD, JA ;

DICK, R ;

SCHEUER, PJ ;

SHERLOCK, S .

GUT, 1980, 21 (10) :870-877

[6]

CHAPMAN VM, 1991, MAMM GENOME, V1, P1

[7]

A SHARED AND UNIQUE EPITOPE(S) ON HUMAN-COLON, SKIN, AND BILIARY EPITHELIUM DETECTED BY A MONOCLONAL-ANTIBODY [J].

DAS, KM ;

VECCHI, M ;

SAKAMAKI, S .

GASTROENTEROLOGY, 1990, 98 (02) :464-469

[8]

NATURAL-HISTORY AND PROGNOSTIC VARIABLES IN PRIMARY SCLEROSING CHOLANGITIS [J].

FARRANT, JM ;

HAYLLAR, KM ;

WILKINSON, ML ;

KARANI, J ;

PORTMANN, BC ;

WESTABY, D ;

WILLIAMS, R .

GASTROENTEROLOGY, 1991, 100 (06) :1710-1717

[9]

RELATIONSHIP OF INFLAMMATORY BOWEL-DISEASE AND PRIMARY SCLEROSING CHOLANGITIS [J].

FAUSA, O ;

SCHRUMPF, E ;

ELGJO, K .

SEMINARS IN LIVER DISEASE, 1991, 11 (01) :31-39

[10]

TOLERANCE TO ENDOTOXIN PREVENTS MORTALITY IN INFECTED THERMAL-INJURY - ASSOCIATION WITH ATTENUATED CYTOKINE RESPONSES [J].

HE, W ;

FONG, YM ;

MARANO, MA ;

GERSHENWALD, JE ;

YURT, RW ;

MOLDAWER, LL ;

LOWRY, SF .

JOURNAL OF INFECTIOUS DISEASES, 1992, 165 (05) :859-864

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