Quantitation of cytomegalovirus (CMV) DNA in leukocytes of human immunodeficiency virus-infected subjects with and without CMV disease by using PCR and the SHARP signal detection system

被引：36

作者：

Boivin, G

Handfield, J

Murray, G

Toma, E

Lalonde, R

Lazar, JG

Bergeron, TG

机构：

[1] UNIV LAVAL,DEPT MICROBIOL,LAVAL,PQ,CANADA

[2] UNIV MONTREAL,DEPT MICROBIOL & IMMUNOL,MONTREAL,PQ H3C 3J7,CANADA

[3] MCGILL UNIV,DEPT MED,MONTREAL,PQ,CANADA

[4] DIGENE DIAGNOST INC,SILVER SPRING,MD

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1997年 / 35卷 / 02期

关键词：

D O I：

10.1128/JCM.35.2.525-526.1997

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We report the development of a simple and rapid PCR assay for quantitation of the cytomegalovirus (CMV) DNA load in polymorphonuclear leukocytes. Using this system, a very good correlation was found between a high number of CMV copies in the blood and the presence of CMV disease in subjects with AIDS.

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页码：525 / 526

页数：2

相关论文

共 15 条

[1] QUANTITATION OF HUMAN CYTOMEGALOVIRUS GLYCOPROTEIN-H GENE IN CELLS USING COMPETITIVE PCR AND A RAPID FLUORESCENCE-BASED DETECTION SYSTEM [J].

BOIVIN, G ;

OLSON, CA ;

QUIRK, MR ;

STCYR, SM ;

JORDAN, MC .

JOURNAL OF VIROLOGICAL METHODS, 1995, 51 (2-3) :329-342

[2] Detection of ganciclovir resistance mutations and quantitation of cytomegalovirus (CMV) DNA in leukocytes of patients with fatal disseminated CMV disease [J].

Boivin, G ;

Chou, SW ;

Quirk, MR ;

Erice, A ;

Jordan, MC .

JOURNAL OF INFECTIOUS DISEASES, 1996, 173 (03) :523-528

[3] DETECTION OF CYTOMEGALOVIRUS (CMV) IN GRANULOCYTES BY POLYMERASE CHAIN-REACTION COMPARED WITH THE CMV ANTIGEN TEST [J].

BOLAND, GJ ;

DEWEGER, RA ;

TILANUS, MGJ ;

VERVERS, C ;

BOSBOOMKALSBEEK, K ;

DEGAST, GC .

JOURNAL OF CLINICAL MICROBIOLOGY, 1992, 30 (07) :1763-1767

[4]

DELFAU MH, 1990, LEUKEMIA, V4, P1

[5] COMPARISON OF SHARP SIGNAL SYSTEM AND SOUTHERN BLOT HYBRIDIZATION ANALYSIS FOR DETECTION OF CYTOMEGALOVIRUS IN CLINICAL SPECIMENS BY PCR [J].

ESPY, MJ ;

SMITH, TF .

JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (11) :3028-3030

[6] QUANTIFICATION OF HUMAN CYTOMEGALOVIRUS DNA USING THE POLYMERASE CHAIN-REACTION [J].

FOX, JC ;

GRIFFITHS, PD ;

EMERY, VC .

JOURNAL OF GENERAL VIROLOGY, 1992, 73 :2405-2408

[7] MONITORING OF HUMAN CYTOMEGALOVIRUS INFECTIONS AND GANCICLOVIR TREATMENT IN HEART-TRANSPLANT RECIPIENTS BY DETERMINATION OF VIREMIA, ANTIGENEMIA, AND DNAEMIA [J].

GERNA, G ;

ZIPETO, D ;

PAREA, M ;

REVELLO, MG ;

SILINI, E ;

PERCIVALLE, E ;

ZAVATTONI, M ;

GROSSI, P ;

MILANESI, G .

JOURNAL OF INFECTIOUS DISEASES, 1991, 164 (03) :488-498

[8] EFFECT OF FOSCARNET INDUCTION TREATMENT ON QUANTITATION OF HUMAN CYTOMEGALOVIRUS (HCMV) DNA IN PERIPHERAL-BLOOD POLYMORPHONUCLEAR LEUKOCYTES AND AQUEOUS-HUMOR OF AIDS PATIENTS WITH HCMV RETINITIS [J].

GERNA, G ;

BALDANTI, F ;

SARASINI, A ;

FURIONE, M ;

PERCIVALLE, E ;

REVELLO, MG ;

ZIPETO, D ;

ZELLA, D ;

CAROSI, GP ;

CASTELLI, F ;

MINOLI, R ;

GROSSI, P ;

MORONI, M ;

DARMINIO, A ;

COPPIN, P ;

ORANI, A ;

BOLIS, D ;

LAZZARIN, A ;

GIANOTTI, N ;

CARNEVALE, G ;

PAN, A ;

SUTER, F ;

PELLEGATA, G ;

FIORI, GP ;

ZEROLI, C ;

CHIODO, F ;

CORONADO, O ;

FIACCADORI, F ;

MAGNANI, G ;

ALBERICI, F ;

VIALE, P ;

GIANNELLI, F ;

GAGGESE, L ;

CADROBBI, P ;

SCAGGIANTE, R ;

CADEO, GP ;

COSTA, P ;

GAFA, S ;

GIUDICI, MG ;

AMBROSOLI, L ;

ANTONIAZZI, E ;

TRIMARCHI, F ;

BERTONI, G ;

BLINI, M ;

DEANGELIS, S ;

LANZA, E ;

VERGANI, A ;

CARRAI, M ;

CAZZOLA, A ;

CERRI, L .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1994, 38 (01) :38-44

[9] Detection of cytomegalovirus in blood donors by PCR using the digene SHARP signal system assay: Effects of sample preparation and detection methodology [J].

Krajden, M ;

Shankaran, P ;

Bourke, C ;

Lau, W .

JOURNAL OF CLINICAL MICROBIOLOGY, 1996, 34 (01) :29-33

[10] QUANTITATION OF HUMAN CYTOMEGALOVIRUS DNA FROM PERIPHERAL-BLOOD CELLS OF HUMAN IMMUNODEFICIENCY VIRUS-INFECTED PATIENTS COULD PREDICT CYTOMEGALOVIRUS RETINITIS [J].

RASMUSSEN, L ;

MORRIS, S ;

ZIPETO, D ;

FESSEL, J ;

WOLITZ, R ;

DOWLING, A ;

MERIGAN, TC .

JOURNAL OF INFECTIOUS DISEASES, 1995, 171 (01) :177-182