Complementary DNA encoding nm23/NDP kinase gene from the Korean tiger shark Scyliorhinus torazame

被引：8

作者：

Cho, JJ

Lee, JH

Kim, SK

Choi, TJ

Kim, YT ^{[1
]}

机构：

[1] Pukyong Natl Univ, Dept Microbiol, Pusan 608737, South Korea

[2] Pukyong Natl Univ, Dept Chem, Pusan 608737, South Korea

来源：

MARINE BIOTECHNOLOGY | 1999年 / 1卷 / 02期

关键词：

tumor metastasis suppressor protein; nucleoside diphosphate kinase; Scyliorhinus torazame; molecular cloning;

D O I：

10.1007/PL00011760

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A new tumor suppressor gene, snm23, homologous to the gene for human nucleoside diphosphate kinase nm23/NDP was first cloned from Korean tiger shark (Scyliorhinus torazame) skin lambda ZAP-II complementary DNA library. The gene (named snm23) containing the tumor metastasis suppressor protein was sequenced. The nucleotide and deduced amino acid sequences of snm23 revealed an open reading frame of 450 bp that corresponded to a protein of 150 amino acid residues, with a calculated molecular mass of 16.8 kDa. Sequence comparison of snm23 with nm23/NDP kinases was performed. In order to determine tissue specificity, reverse transcription-polymerase chain reaction was used. The expression of snm23/NDP kinase was detected in tissues from skin, cartilage, and liver of Korean tiger shark.

引用

页码：131 / 136

页数：6

共 40 条

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