Relationship between outer membrane protein and lipopolysaccharide profiles and serotypes of enterotoxigenic Escherichia coli isolated in Brazil

被引:17
作者
Nishimura, LS
Ferreira, LCS
Pacheco, ABF
Guth, BEC
机构
[1] UNIV FED SAO PAULO,ESCOLA PAULISTA MED,DEPT MICROBIOL IMMUNOL & PARASITOL,BR-04023062 SAO PAULO,BRAZIL
[2] FED UNIV RIO DE JANEIRO,CCS,INST BIOFIS CARLOS CHAGAS FILHO,BR-21941590 RIO JANEIRO,BRAZIL
基金
巴西圣保罗研究基金会;
关键词
enterotoxigenic Escherichia coli; serotype; outer membrane protein; lipopolysaccharide; clonal grouping; Escherichia coli;
D O I
10.1016/0378-1097(96)00322-9
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The electrophoretic profiles of outer membrane proteins and lipopolysaccharide of sixty-five enterotoxigenic Escherichia coli of different serotypes and virulence-associated factors, toxin and colonization factors were determined. A close relationship between serotype and outer membrane protein and lipopolysaccharide patterns could be observed. No correlation could be found between the electrophoretic profiles and the expression of virulence-associated factors. The observed homogeneity of outer membrane protein and lipopolysaccharide profiles suggested the presence of only a few clones in the samples studied, and supported the use of outer membrane protein and lipopolysaccharide analysis as a useful epidemiological tool in the characterization of enterotoxigenic Escherichia coli isolates.
引用
收藏
页码:253 / 258
页数:6
相关论文
共 25 条
[1]   CLONAL ANALYSIS OF ESCHERICHIA-COLI O2-K1 ISOLATED FROM DISEASED HUMANS AND ANIMALS [J].
ACHTMAN, M ;
HEUZENROEDER, M ;
KUSECEK, B ;
OCHMAN, H ;
CAUGANT, D ;
SELANDER, RK ;
VAISANENRHEN, V ;
KORHONEN, TK ;
STUART, S ;
ORSKOV, F ;
ORSKOV, I .
INFECTION AND IMMUNITY, 1986, 51 (01) :268-276
[2]  
ACHTMAN M, 1986, ANNU REV MICROBIOL, V40, P185, DOI 10.1146/annurev.mi.40.100186.001153
[3]   LIPOPOLYSACCHARIDE PROFILE TYPING AS A TECHNIQUE FOR COMPARATIVE TYPING OF GRAM-NEGATIVE BACTERIA [J].
AUCKEN, HM ;
PITT, TL .
JOURNAL OF CLINICAL MICROBIOLOGY, 1993, 31 (05) :1286-1289
[4]  
BLACK RE, 1990, REV INFECT DIS, V12, pS73
[5]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[6]   COLONIZATION FACTORS OF DIARRHEAGENIC ESCHERICHIA-COLI AND THEIR INTESTINAL RECEPTORS [J].
CASSELS, FJ ;
WOLF, MK .
JOURNAL OF INDUSTRIAL MICROBIOLOGY, 1995, 15 (03) :214-226
[7]  
CHERIFI A, 1994, J CLIN MICROBIOL, V32, P1197
[8]   CLONAL RELATIONSHIPS AMONG ESCHERICHIA-COLI SEROGROUP-06 ISOLATES FROM HUMAN AND ANIMAL INFECTIONS [J].
CHERIFI, A ;
CONTREPOIS, M ;
PICARD, B ;
GOULLET, P ;
ORSKOV, I ;
ORSKOV, F ;
DERYCKE, J .
FEMS MICROBIOLOGY LETTERS, 1991, 80 (2-3) :225-230
[9]   Intra-specific diversity within Pasteurella trehalosi based on variation of capsular polysaccharide, lipopolysaccharide and outer-membrane proteins [J].
Davies, RL ;
Quirie, M .
MICROBIOLOGY-SGM, 1996, 142 :551-560
[10]   EVALUATION OF ANTISERA USED FOR DETECTING ENTERO-TOXIGENIC ESCHERICHIA-COLI IN SAO-PAULO [J].
GUTH, BEC ;
TRABULSI, LR .
JOURNAL OF CLINICAL MICROBIOLOGY, 1985, 22 (04) :626-628