Synthesis and testing of a novel fluorescence label for carboxyls in carbohydrates and cellulosics

被引：15

作者：

Bohrn, R

Potthast, A

Rosenau, T

Sixta, H

Kosma, P

机构：

[1] Univ Nat Resources & Appl Life Sci, Dept Chem, A-1190 Vienna, Austria

[2] Univ Nat Resources & Appl Life Sci, Christian Doppler Lab, A-1190 Vienna, Austria

[3] Lenzing AG, Res & Dev, A-4860 Lenzing, Austria

来源：

SYNLETT | 2005年 / 20期

关键词：

carboxyls; polysaccharides; uronic acids; fluorescence labeling; carbohydrates;

D O I：

10.1055/s-2005-921923

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

A carboxyl-selective fluorescence label, 9H-fluoren-2-yl-diazomethane (FDAM, 6) for the detection of carboxyl groups specifically in polysaccharides and low-molecular weight carbohydrates has been developed. Attached in a pre-column derivatization step, the label allows detection of minute amounts of oxidized functionalities in oligosaccharides and cellulosics under the relatively harsh conditions of GPC separation in the DMAc/LiCl eluant and without any fluorescence interference with light scattering (MALLS) detection, so that carboxyl profiles of polysaccharides were obtained for the first time. Synthetic carbohydrate model compounds (1-3) were used to optimize the labeling reaction.

引用

页码：3087 / 3090

页数：4

共 17 条

[1] 9-DIAZOMETHYLANTHRACENE AS A NEW FLUORESCENCE AND ULTRAVIOLET LABEL FOR THE SPECTROMETRIC DETECTION OF PICOMOLE QUANTITIES OF FATTY-ACIDS BY HIGH-PRESSURE LIQUID-CHROMATOGRAPHY [J].