Development and evaluation of an immunomagnetic separation-ELISA for the detection of Staphylococcus aureus thermostable nuclease in composite milk

被引：37

作者：

Yazdankhah, SP

Solverod, L

Simonsen, S

Olsen, E

机构：

[1] Norwegian Coll Vet Med, Dept Pharmacol Microbiol & Food Hyg, N-0033 Oslo, Norway

[2] Mastitis Lab, N-6401 Oslo, Norway

[3] Dynal AS, N-0212 Oslo, Norway

来源：

VETERINARY MICROBIOLOGY | 1999年 / 67卷 / 02期

关键词：

Staphylococcus aureus; mastitis; composite milk; monodisperse magnetic beads; ELISA;

D O I：

10.1016/S0378-1135(99)00035-8

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

An ELISA method based on monodisperse magnetic beads was developed for the detection of Staphylococcus aureus thermostable nuclease (TNase) in composite milk, wherein S. aureus TNase is captured by magnetic beads coated with monoclonal antibodies directed against TNase and subsequently detected by an enzyme-labelled MAb against the same antigen. Sensitivity of the test was approximately 1 ng TNase, which corresponds to the amount of TNase produced and secreted by approximately 10(5) S. aureus per mi. The Immune Magnetic Separation (IMS)-ELISA detected TNase in samples from which no S. nul-ells could be demonstrated on culture. The total test time is 3 h and can be performed either on preserved or fresh milk. The method may be automated. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：113 / 125

页数：13

共 41 条

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