On the osmotically unresponsive water compartment in cells

被引：17

作者：

Fullerton, GD

Kanal, KM

Cameron, IL

机构：

[1] Univ Texas, Hlth Sci Ctr, Dept Radiol, San Antonio, TX 78285 USA

[2] Univ Texas, Hlth Sci Ctr, Dept Cell & Struct Biol, San Antonio, TX 78285 USA

来源：

CELL BIOLOGY INTERNATIONAL | 2006年 / 30卷 / 01期

关键词：

osmosis; interfacial water; osmotically unresponsive water; cell water;

D O I：

10.1016/j.cellbi.2005.09.007

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Differences in colligative properties (freezing point, boiling point, vapor pressure and osmotic behavior) between water in living cells and pure bulk water were investigated by re-evaluating reports of the osmotic behavior of mammalian cells. In five different animal cells, osmotically unresponsive water (OUW) values ranged from 1.1 to 2.2 g per a dry mass. Detailed analysis of human red blood cell (RBC) data indicates a major role for hemoglobin OUW-values, aggregation and packing in cell volume regulation that can be explained for the first time in relevant molecular terms. (c) 2005 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.

引用

收藏

页码：74 / 77

页数：4

相关论文

共 17 条

[1] A theory of partial osmotic pressures and membrane equilibria, with special reference to the application of Dalton's law to h ae moglobin solutions in the presence of salts [J].

Adair, GS .

PROCEEDINGS OF THE ROYAL SOCIETY OF LONDON SERIES A-CONTAINING PAPERS OF A MATHEMATICAL AND PHYSICAL CHARACTER, 1928, 120 (786) :573-603

[2] Augmented water binding and low cellular water content in erythrocytes of camel and camelids [J].

Bogner, P ;

Csutora, P ;

Cameron, IL ;

Wheatley, DN ;

Miseta, A .

BIOPHYSICAL JOURNAL, 1998, 75 (06) :3085-3091

[3]

BOGNER P, 2005, IN PRESS CELL BIOL I

[4] Role of protein conformation and aggregation in pumping water in and out of a cell [J].

Cameron, IL ;

Kanal, KM ;

Fullerton, GD .

CELL BIOLOGY INTERNATIONAL, 2006, 30 (01) :78-85

[5] MAINTENANCE AND MOBILITY OF HEMOGLOBIN AND WATER WITHIN THE HUMAN ERYTHROCYTE AFTER DETERGENT DISRUPTION OF THE PLASMA-MEMBRANE [J].

CAMERON, IL ;

COX, LA ;

LIU, XR ;

FULLERTON, GD .

JOURNAL OF CELLULAR PHYSIOLOGY, 1991, 149 (03) :365-374

[6] EXTENT AND PROPERTIES OF NONBULK BOUND WATER IN CRYSTALLINE LENS CELLS [J].

CAMERON, IL ;

CONTRERAS, E ;

FULLERTON, GD ;

KELLERMAYER, M ;

LUDANY, A ;

MISETA, A .

JOURNAL OF CELLULAR PHYSIOLOGY, 1988, 137 (01) :125-132

[7] OSMOTIC AND MOTIONAL PROPERTIES OF INTRACELLULAR WATER AS INFLUENCED BY OSMOTIC SWELLING AND SHRINKAGE OF XENOPUS OOCYTES [J].

CAMERON, IL ;

MERTA, P ;

FULLERTON, GD .

JOURNAL OF CELLULAR PHYSIOLOGY, 1990, 142 (03) :592-602

[8] A MODEL TO EXPLAIN THE OSMOTIC-PRESSURE BEHAVIOR OF HEMOGLOBIN AND SERUM-ALBUMIN [J].

CAMERON, IL ;

FULLERTON, GD .

BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE, 1990, 68 (05) :894-898

[9] Osmotically unresponsive water fraction on proteins: Non-ideal osmotic pressure of bovine serum albumin as a function of pH and salt concentration [J].

Fullerton, GD ;

Kanal, KM ;

Cameron, IL .

CELL BIOLOGY INTERNATIONAL, 2006, 30 (01) :86-92

[10] METHOD TO IMPROVE THE ACCURACY OF MEMBRANE OSMOMETRY MEASURES OF PROTEIN MOLECULAR-WEIGHT [J].

FULLERTON, GD ;

ZIMMERMAN, RJ ;

KANAL, KM ;

FLOYD, LJ ;

CAMERON, IL .

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS, 1993, 26 (04) :299-307