Ultra-performance liquid chromatography coupled to linear ion trap mass spectrometry for the identification of drug metabolites in biological samples

被引：25

作者：

Dear, GJ ^{[1
]}

James, AD ^{[1
]}

Sarda, S ^{[1
]}

机构：

[1] GlaxoSmithKline Inc, Sturctural Identificat, DMPK, Ware SG12 0DP, Herts, England

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 2006年 / 20卷 / 08期

关键词：

D O I：

10.1002/rcm.2462

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The coupling of ultra-performance liquid chromatography, operating at elevated pressures, to a linear ion trap mass spectrometer provides a high-performance system suitable for drug metabolite characterisation. This system demonstrates improved chromatographic efficiency and sensitivity and at the same time provides diagnostic MSn data often critical for metabolite structural assignment. The linear ion trap was capable of dealing with the high chromatographic efficiencies and hence narrow peak widths associated with 1.7 mu m particle-packed column separations. Polarity switching and data-dependent MSn data were generated with ease, and applied to the identification of metabolites found in human plasma. Copyright (c) 2006 John Wiley & Sons, Ltd.

引用

页码：1351 / 1360

页数：10

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