Lithoheterotrophic growth and electron transfer chain components of the filamentous gliding bacterium Leucothrix mucor DSM 2157 during oxidation of sulfur compounds

被引:20
作者
Grabovich, MY
Muntyan, MS
Lebedeva, VY
Ustiyan, VS
Dubinina, GA
机构
[1] Russian Acad Sci, Inst Microbiol, Lab Ecol & Geochem Act Microorganisms, Moscow 117811, Russia
[2] Voronezh State Univ, Dept Plant Biochem & Physiol, Voronezh 394693, Russia
[3] Moscow MV Lomonosov State Univ, AN Belozersky Inst Physicochem Biol, Dept Bioenerget, Moscow 119899, Russia
基金
俄罗斯基础研究基金会;
关键词
colorless sulfur bacterium; lithoheterotrophy; cytochrome; sulfur metabolism; metabolic regulation; Leucothrix mucor;
D O I
10.1016/S0378-1097(99)00325-0
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Evidence is presented that the type strain of filamentous gliding bacterium Leucothrix mucor DSM 2157 is capable of lithoheterotrophic growth. Thiosulfate oxidation was accompanied by accumulation of sulfate and tetrathionate in the growth medium and intracellular accumulation of elemental sulfur, as occurs in filamentous sulfur bacteria of the genus Thiothrix. Thiosulfate oxidation by L. mucor was induced during growth with a limiting concentration of organic substrate (i.e. 50-170 mg l(-1) tryptone) and resulted from the induction of dissimilatory enzymes of sulfur metabolism. Metabolically useful energy was derived by both substrate-linked and oxidative phosphorylation from thiosulfate and sulfite oxidation. During sulfite oxidation the electron transfer pathway was shown to begin at the ubiquinone-cytochrome b segment of the electron transport chain. L. mucor limited by organic substrate had lowered levels of membrane bound cytochromes c and respiratory activity but a higher input of a cyanide-insensitive respiratory component than during organic enriched growth. Cytochromes aa(3) and d, which had not been shown previously in this bacterium, were detected in the cells under various growth conditions. Cytochrome aa(3) is suggested to take part in the electron transfer pathway from thiosulfate to oxygen which was completely inhibited by 100 mu M cyanide. (C) 1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
引用
收藏
页码:155 / 161
页数:7
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