The expression of genes in the ubiquitin-proteasome proteolytic pathway is increased in skeletal muscle from patients with cancer

被引：139

作者：

Williams, A ^{[1
]}

Sun, XY ^{[1
]}

Fischer, JE ^{[1
]}

Hasselgren, PO ^{[1
]}

机构：

[1] Univ Cincinnati, Dept Surg, Cincinnati, OH 45267 USA

来源：

SURGERY | 1999年 / 126卷 / 04期

关键词：

D O I：

10.1016/S0039-6060(99)70131-5

中图分类号：

R61 [外科手术学];

学科分类号：

摘要：

Background: The intracellular mechanisms of muscle cachexia in patients with cancer are not known. To assess the role of the ubiquitin-proteasome proteolytic pathway in cancer-induced muscle breakdown, we determined messenger RNA levels for ubiquitin and several 20S proteasome subunits in muscle from patients undergoing surgery for cancer. Methods: A biopsy specimen was obtained from the rectus abdominis muscle in patients undergoing laparotomy for cancer (n = 6) or noncancer disease (n = 6). Tissue levels of mRNA for ubiquitin and the 20S proteasome subunits HC3, HC5, HC7, and HC9 were determined by dot blot analysis. Results: The mRNA levels for ubiquitin and the 20S proteasome subunits were 2 to 4 times higher in muscle from patients with cancer than in muscle from control patients. Conclusion: This is the first report of increased expression of genes in the ubiquitin-proteasome proteolytic pathway in muscle tissue from patients with cancer. Cancer-induced muscle catabolism may at least in part reflect ubiquitin-proteasome-dependent protein breakdown.

引用

页码：744 / 749

页数：6

共 25 条

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