Isolation and sequencing of porcine fast skeletal muscle alkali myosin light chain 3 cDNA

被引:6
作者
Davoli, R
Fontanesi, L
Costosi, E
Zambonelli, P
Russo, V
机构
[1] DIPROVAL, Sezione di Allevamenti Zootecnici, University of Bologna
关键词
D O I
10.1080/10495399709525880
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
A cDNA coding for alkali myosin light chain 3 (MLC3(F)) was isolated from a porcine skeletal muscle library. This clone has an insert of 859 bp encompassing the complete CDS (coding sequence) plus the 5' and 3' untranslated regions. Computer analysis showed that porcine MLC3, cDNA is highly homologous to the corresponding cDNAs of human, rabbit and rat. Moreover, Northern analysis showed the presence of two bands that represent the mature mRNAs of the MLClF and MLC3(F) isoforms according to data observed in other species.
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页码:179 / 185
页数:7
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