Expression of green fluorescent protein as a marker for effects of antileishmanial compounds in vitro

被引：38

作者：

Kamau, SW ^{[1
]}

Grimm, F ^{[1
]}

Hehl, AB ^{[1
]}

机构：

[1] Univ Zurich, Inst Parasitol, CH-8057 Zurich, Switzerland

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2001年 / 45卷 / 12期

关键词：

D O I：

10.1128/AAC.45.12.3654-3656.2001

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Transgenic Leishmania infantum promastigotes, which constitutively express green fluorescent protein (GFP) in their cytoplasm, were used to monitor the effects of antileishmanial compounds in real time. The GFP-based assay provided a reliable measure of drug-induced inhibitory effects on protein expression, resulting in a dynamic picture of the responses of leishmanial promastigotes to the compounds tested.

引用

页码：3654 / 3656

页数：3

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