Determination of monomethylarsonic acid and dimethylarsinic acid in urine by liquid chromatography-tandem mass spectrometry

被引：9

作者：

Le Bouil, A ^{[1
]}

Cailleux, A ^{[1
]}

Turcant, A ^{[1
]}

Allain, P ^{[1
]}

机构：

[1] CHU Angers, Lab Pharmacol & Toxicol, F-49033 Angers, France

来源：

JOURNAL OF ANALYTICAL TOXICOLOGY | 1999年 / 23卷 / 04期

关键词：

D O I：

10.1093/jat/23.4.257

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A method for the simultaneous measurement of monomethylarsonic acid (MMA) and dimethylarsinic acid (DMA) in human urine using liquid chromatography-tandem mass spectrometry with electrospray ionization (LC-ES- MS-MS) was developed. The multiple reaction monitoring mode (MRM) was used for quantitation. The protonated molecule ions (m/z 141.0 for MMA and m/z 139.0 for DMA) were selected as precursor ions, and the same fragment ion AsO+ (m/z 91.1) was monitored as the product ion. A two-step liquid-liquid extraction of MMA and DMA from urine provided recoveries of 92-100%. The coefficients of variation were lower than 7% for the within-day precision and lower than 11% for the between-day precision. The limit of quantitation was 25 μg/L as As for the two analytes. The assay was linear over the range of 25-800 μg/L.

引用

页码：257 / 261

页数：5

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