Salt tolerance of transgenic rice overexpressing yeast mitochondrial Mn-SOD in chloroplasts

被引：141

作者：

Tanaka, Y

Hibino, T

Hayashi, Y

Tanaka, A

Kishitani, S

Takabe, T ^{[1
]}

Yokota, S

Takabe, T ^{[1
]}

机构：

[1] Meijo Univ, Fac Sci & Technol, Dept Chem, Tenpaku Ku, Nagoya, Aichi 4688502, Japan

[2] Mitsubishi Chem Corp, Plant Biotech Res Inst, Midori Ku, Kanagawa 2770033, Japan

[3] Tohoku Univ, Grad Sch Agr Sci, Lab Plant Breeding & Genet, Aoba Ku, Kanagawa 2770033, Japan

[4] Nagoya Univ, Sch Agr Sci, Biosci Ctr, Chikusa Ku, Nagoya, Aichi 4648601, Japan

[5] Yamanaski Med Coll, Dept Anat, Yamanashi 4093821, Japan

[6] Meijo Univ, Inst Res, Tenpaku Ku, Nagoya, Aichi 4688502, Japan

来源：

PLANT SCIENCE | 1999年 / 148卷 / 02期

基金：

日本学术振兴会;

关键词：

rice; Mn-SOD; oxidative stress; salt tolerance; transgenic rice;

D O I：

10.1016/S0168-9452(99)00133-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The potential role of superoxide dismutase (SOD) in the protection against salt stress was examined using transgenic rice plants. The coding region of the yeast mitochondrial Mn-SOD gene was fused with the chloroplast targetting signal of glutamine synthetase gene and introduced into rice protoplasts by electroporation. Immunogold labeling experiments revealed that the yeast Mn-SOD was accumulated in the chloroplasts of transgenic rice. The total SOD activity in the control plant was mostly attributed to the activity of cytosolic and chloroplastic Cu/Zn-SOD. Total SOD activity in the transformant was about 1.7-fold that of the control under non-stressed conditions. The photosynthetic electron transport rates of control and transgenic rice were similar under non-stressed conditions. Upon salt stress (100 mM NaCl), the SOD activities decreased in both plants, but the decrease was faster in the control plant. The activities of overexpressed Mn-SOD and cytosolic Cu/Zn-SOD did not change upon salt stress in either the transgenic or control plants, whereas the chloroplastic Cu/Zn-SOD activity in control rice decreased significantly. At high salinity, the ascorbate peroxidase activity of the transformant was about 1.5-fold higher than that in the control. These results suggest that increased levels of ascorbate peroxidase and high levels of chloroplastic SOD in the transformant are important factors for salt resistance in rice. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：131 / 138

页数：8

共 31 条

[1]

Asada K., 1994, Causes of photooxidative stress and amelioration of defense systems in plants., P77

[2] ISOENZYMES OF SUPEROXIDE DISMUTASE FROM WHEAT-GERM [J].