Rapid photolytic release of cytidine 5-diphosphate from a coumarin derivative:: A new tool for the investigation of ribonucleotide reductases

被引：57

作者：

Schönleber, RO

Bendig, J

Hagen, V

Giese, B

机构：

[1] Humboldt Univ, Inst Chem, D-10115 Berlin, Germany

[2] Univ Basel, Dept Chem, CH-4056 Basel, Switzerland

[3] Res Inst Mol Pharmacol, D-10315 Berlin, Germany

来源：

BIOORGANIC & MEDICINAL CHEMISTRY | 2002年 / 10卷 / 01期

关键词：

D O I：

10.1016/S0968-0896(01)00254-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In order to study the range-range radical transfer in the Escherichia coli ribonucleotide reductase (RNR), caged cytidine 5'-diphospliate (CDP) 1 was synthesized, which contains the photolabile (7-diethylaminocoumarin-4-yl)methyl moiety. The caged CDP 1 triggers the release of CDP when irradiated at wavelengths between 365 and 436 nm. The rate constant of the formation of alcohol 2 and cytidine 5'-diphosphate 3 is 2 x 10(8) s(-1) and the quantum efficiency for the disappearance of caged CDP 1 is 2.9%. (C) 2001 Elsevier Science Ltd. All rights reserved.

引用

页码：97 / 101

页数：5

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