Hydrogen peroxide is a regulator of ABI1, a protein phosphatase 2C from Arabidopsis

被引：154

作者：

Meinhard, M ^{[1
]}

Grill, E ^{[1
]}

机构：

[1] Tech Univ Munich, Lehrstuhl Bot, Biologikum Weihenstephan, D-85354 Freising Weihenstephan, Germany

来源：

FEBS LETTERS | 2001年 / 508卷 / 03期

关键词：

abscisic acid; protein phosphatase 2C; plant hormone; signalling; Arabidopsis;

D O I：

10.1016/S0014-5793(01)03106-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Protein phosphatases 2C (PP2Cs) exhibit diverse regulatory functions in signalling pathways of animals, yeast and plants. ABI1 is a PP2C of Arabidopsis that exerts negative control on signalling of the phytohormone abscisic acid (ABA). Characterisation of the redox sensitivity of ABI1 revealed a strong enzymatic inactivation by hydrogen peroxide (H2O2) which has recently been implicated as a secondary messenger of ABA signalling. H2O2 reversibly inhibited ABI1 activity in vitro with an IC50 of approximately 140 muM in the presence of physiological concentrations of glutathione. In addition, ABI1 was highly susceptible to inactivation by phenylarsine oxide (IC50 = 3-4 muM) indicative for the facile oxidation of vicinal cysteine residues. Thus, H2O2 generated during ABA signalling seems to inactivate the negative regulator of the ABA response. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：443 / 446

页数：4

共 54 条

[1] Two distinct sources of elicited reactive oxygen species in tobacco epidermal cells [J].

Allan, AC ;

Fluhr, R .

PLANT CELL, 1997, 9 (09) :1559-1572

[2] Reactive oxygen intermediates mediate a systemic signal network in the establishment of plant immunity [J].

Alvarez, ME ;

Pennell, RI ;

Meijer, PJ ;

Ishikawa, A ;

Dixon, RA ;

Lamb, C .

CELL, 1998, 92 (06) :773-784

[3] Epidermal growth factor (EGF)-induced generation of hydrogen peroxide - Role in EGF receptor-mediated tyrosine phosphorylation [J].

Bae, YS ;

Kang, SW ;

Seo, MS ;

Baines, IC ;

Tekle, E ;

Chock, PB ;

Rhee, SG .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (01) :217-221

[4] Roles of superoxide radical anion in signal transduction mediated by reversible regulation of protein-tyrosine phosphatase 1B [J].

Barrett, WC ;

DeGnore, JP ;

Keng, YF ;

Zhang, ZY ;

Yim, MB ;

Chock, PB .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (49) :34543-34546

[5] Unsaturated fatty acids inhibit MP2C, a protein phosphatase 2C involved in the wound-induced MAP kinase pathway regulation [J].

Baudouin, E ;

Meskiene, I ;

Hirt, H .

PLANT JOURNAL, 1999, 20 (03) :343-348

[6] Protein phosphatase activity of abscisic acid insensitive 1 (ABI1) protein from Arabidopsis thaliana [J].

Bertauche, N ;

Leung, J ;

Giraudat, J .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1996, 241 (01) :193-200

[7] Inactivation of calcineurin by hydrogen peroxide and phenylarsine oxide - Evidence for a dithiol-disulfide equilibrium and implications for redox regulation [J].

Bogumil, R ;

Namgaladze, D ;

Schaarschmidt, D ;

Schmachtel, T ;

Hellstern, S ;

Mutzel, R ;

Ullrich, V .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 2000, 267 (05) :1407-1415

[8] The inactivation mechanism of low molecular weight phosphotyrosine-protein phosphatase by H2O2 [J].

Caselli, A ;

Marzocchini, R ;

Camici, G ;

Manao, G ;

Moneti, G ;

Pieraccini, G ;

Ramponi, G .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (49) :32554-32560

[9] Two vicinal cysteines confer a peculiar redox regulation to low molecular weight protein tyrosine phosphatase in response to platelet-derived growth factor receptor stimulation [J].

Chiarugi, P ;

Fiaschi, T ;

Taddei, ML ;

Talini, D ;

Giannoni, E ;

Raugei, G ;

Ramponi, G .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (36) :33478-33487

[10] THE STRUCTURE AND REGULATION OF PROTEIN PHOSPHATASES [J].

COHEN, P .

ANNUAL REVIEW OF BIOCHEMISTRY, 1989, 58 :453-508

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