Saccharomyces cerevisiae mitoproteome plasticity in response to recombinant alternative ubiquinol oxidase

被引：13

作者：

Mathy, G

Navet, R

Gerkens, P

Leprince, P

De Pauw, E

Sluse-Goffart, CM

Sluse, FE

Douette, P

机构：

[1] Ctr Rech & Neurobiol Cellulaire & Mol, Lab Bioenerget, B-4000 Liege, Belgium

[2] Ctr Rech & Neurobiol Cellulaire & Mol, Lab Mass Spect, B-4000 Liege, Belgium

来源：

JOURNAL OF PROTEOME RESEARCH | 2006年 / 5卷 / 02期

关键词：

alternative oxidase; yeast; mitoproteome; proteomics; two-dimensional differential in-gel electrophoresis;

D O I：

10.1021/pr050346e

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The energy-dissipating alternative oxidase (AOX) from Hansenula anomala, was expressed in Saccharomyces cerevisiae. The recombinant AOX was functional. A comparative analysis by two-dimensional differential in-gel electrophoresis (2D-DIGE) of mitochondrial protein patterns found in wild-type and recombinant AOX strains was performed. 60 proteins exhibiting a significant difference in their abundance were identified. Interestingly, proteins implicated in major metabolic pathways such as Krebs cycle and amino acid biosynthesis were up-regulated. Surprisingly, an up-regulation of the respiratory-chain complex III was associated with a down-regulation of the ATP synthase complex.

引用

页码：339 / 348

页数：10

共 44 条

[1] Functional expression of the plant alternative oxidase affects growth of the yeast Schizosaccharomyces pombe [J].