Optogenetic protein clustering and signaling activation in mammalian cells

被引：339

作者：

Bugaj, Lukasz J. ^{[1
,2
]}

Choksi, Atri T. ^{[1
]}

Mesuda, Colin K. ^{[3
]}

Kane, Ravi S. ^{[4
,5
]}

Schaffer, David V. ^{[1
,2
,3
,6
,7
]}

机构：

[1] Univ Calif Berkeley, Dept Bioengn, Berkeley, CA 94720 USA

[2] Univ Calif Berkeley, UC Berkeley UCSF Grad Program Bioengn, Berkeley, CA 94720 USA

[3] Univ Calif Berkeley, Dept Chem Engn, Berkeley, CA 94720 USA

[4] Rensselaer Polytech Inst, Howard P Isermann Dept Chem & Biol Engn, Troy, NY 12180 USA

[5] Rensselaer Polytech Inst, Ctr Biotechnol & Interdisciplinary Studies, Troy, NY USA

[6] Univ Calif Berkeley, Helen Wills Neurosci Inst, Berkeley, CA 94720 USA

[7] Univ Calif Berkeley, Calif Inst Quantitat Biosci, Berkeley, CA 94720 USA

来源：

NATURE METHODS | 2013年 / 10卷 / 03期

关键词：

SPATIOTEMPORAL CONTROL; INDUCTION;

D O I：

10.1038/nmeth.2360

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We report an optogenetic method based on Arabidopsis thaliana cryptochrome 2 for rapid and reversible protein oligomerization in response to blue light. We demonstrated its utility by photoactivating the beta-catenin pathway, achieving a transcriptional response higher than that obtained with the natural ligand Wnt3a. We also demonstrated the modularity of this approach by photoactivating RhoA with high spatiotemporal resolution, thereby suggesting a previously unknown mode of activation for this Rho GTPase.

引用

页码：249 / 252

页数：4

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