Adhesion, growth and detachment of cells on modified polystyrene surface

被引:27
作者
Hendrick, V [1 ]
Muniz, E [1 ]
Geuskens, G [1 ]
Wérenne, J [1 ]
机构
[1] Free Univ Brussels, B-1050 Brussels, Belgium
关键词
adsorption of poly(N-isopropylarcrylamide); cell adhesion; surface modification of polystyrene; temperature dependent detachment;
D O I
10.1023/A:1014041003617
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
By adsorbing poly(N-isopropylacrylamide) (PNIPAAm) from an aqueous solution onto oxidised polystyrene without the need for grafting the polymer to the surface, we showed here that cells (CHO-K1) adhere and grow well at 37 C and are detached by lowering the temperature to 10degreesC without any other deleterious treatment. Both bacterial culture grade polystyrene Petri dishes and polystyrene beads (120 to 250 mum diamaters) commercially available used in static conditions of growth were tested with similar results. The contact angle of modified Petri dishes with a water droplet increases from 36 to 58degrees when the temperature is raised from 25 to 37degreesC indicating change in hydrophilicity of the surface as a function of temperature.
引用
收藏
页码:49 / 53
页数:5
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