Perspective - Living cells as test tubes

被引：204

作者：

Xie, XS ^{[1
]}

Yu, J ^{[1
]}

Yang, WY ^{[1
]}

机构：

[1] Harvard Univ, Dept Chem & Biol Chem, Cambridge, MA 02138 USA

来源：

SCIENCE | 2006年 / 312卷 / 5771期

关键词：

D O I：

10.1126/science.1127566

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The combination of specific probes and advanced optical microscopy now allows quantitative probing of biochemical reactions in living cells. On selected systems, one can detect and track a particular protein with single-molecule sensitivity, nanometer spatial precision, and millisecond time resolution. Metabolites, usually difficult to detect, can be imaged and monitored in living cells with coherent anti-Stokes Raman scattering microscopy. Here, we describe the application of these techniques in studying gene expression, active transport, and lipid metabolism.

引用

页码：228 / 230

页数：3

共 31 条

[1] FORCE GENERATION OF ORGANELLE TRANSPORT MEASURED INVIVO BY AN INFRARED-LASER TRAP [J].

ASHKIN, A ;

SCHUTZE, K ;

DZIEDZIC, JM ;

EUTENEUER, U ;

SCHLIWA, M .

NATURE, 1990, 348 (6299) :346-348

[2] Using single-particle tracking to study nuclear trafficking of viral genes [J].

Babcock, HP ;

Chen, C ;

Zhuang, XW .

BIOPHYSICAL JOURNAL, 2004, 87 (04) :2749-2758

[3] PROPOSED METHOD FOR MOLECULAR OPTICAL IMAGING [J].

BETZIG, E .

OPTICS LETTERS, 1995, 20 (03) :237-239

[4] tRNA dynamics on the ribosome during translation [J].

Blanchard, SC ;

Kim, HD ;

Gonzalez, RL ;

Puglisi, JD ;

Chu, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (35) :12893-12898

[5] Semiconductor nanocrystals as fluorescent biological labels [J].

Bruchez, M ;

Moronne, M ;

Gin, P ;

Weiss, S ;

Alivisatos, AP .

SCIENCE, 1998, 281 (5385) :2013-2016

[6] Stochastic protein expression in individual cells at the single molecule level [J].

Cai, L ;

Friedman, N ;

Xie, XS .

NATURE, 2006, 440 (7082) :358-362

[7] Quantum dot bioconjugates for ultrasensitive nonisotopic detection [J].

Chan, WCW ;

Nie, SM .

SCIENCE, 1998, 281 (5385) :2016-2018

[8] Coherent anti-Stokes Raman scattering microscopy: Instrumentation, theory, and applications [J].

Cheng, JX ;

Xie, XS .

JOURNAL OF PHYSICAL CHEMISTRY B, 2004, 108 (03) :827-840

[9] Single-molecule study of transcriptional pausing and arrest by E-coli RNA polymerase [J].

Davenport, RJ ;

Wuite, GJL ;

Landick, R ;

Bustamante, C .

SCIENCE, 2000, 287 (5462) :2497-2500

[10] Visualization of the movement of single histidine kinase molecules in live Caulobacter cells [J].

Deich, J ;

Judd, EM ;

McAdams, HH ;

Moerner, WE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (45) :15921-15926

← 1 2 3 4 →