Method for estimation of proanthocyanidins based on their acid depolymerization in the presence of nucleophiles

被引：140

作者：

Matthews, S

Mila, I

Scalbert, A

Pollet, B

Lapierre, C

duPenhoat, CLMH

Rolando, C

Donnelly, DMX

机构：

[1] INRA, CHIM BIOL LAB, INST NATL AGRON PARIS GRIGNON, F-78850 THIVERVAL GRIGNON, FRANCE

[2] ECOLE NORMALE SUPER, DEPT CHIM, URA 1679, F-75231 PARIS 05, FRANCE

[3] NATL UNIV IRELAND UNIV COLL DUBLIN, DEPT CHEM, DUBLIN 4, IRELAND

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 1997年 / 45卷 / 04期

关键词：

proanthocyanidins; tannins; estimation; thiolysis; phloroglucinol; POLYMERIC PROANTHOCYANIDINS; LIQUID-CHROMATOGRAPHY; CONDENSED TANNINS; MOLECULAR-WEIGHT; PROCYANIDINS; UNITS; BARK;

D O I：

10.1021/jf9607573

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Depolymerization of proanthocyanidins in the presence of phloroglucinol or benzyl mercaptan nucleophiles was studied to develop a method for their quantitative determination. Degradation products were separated and quantified by either GC or HPLC and the reaction conditions optimized. The high depolymerization yields obtained with benzyl mercaptan make the method suitable for proanthocyanidin determination. The method is more specific and sensitive than the colorimetric methods classically used. It is particularly well suited for the analysis of samples containing either low amounts of proanthocyanidins or insoluble proanthocyanidins.

引用

页码：1195 / 1201

页数：7

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