Determination of atrazine in soil samples by ELISA using polyclonal and monoclonal antibodies

被引:9
作者
Deng, AP [1 ]
Fránek, M [1 ]
Kolár, V [1 ]
机构
[1] Vet Res Inst, CS-62132 Brno, Czech Republic
关键词
ELISA; atrazine; antibodies; screening; gas chromatography; soil;
D O I
10.1080/09540109999816
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
Atrazine concentrations were measured by polyclonal antibody (PAb) and monoclonal antibody (MAb) ELISAs in methanolic extracts of both laboratory-fortified and field samples collected from 12 maize fields. Analysis of 91 soil samples collected in the Southern Moravian region demonstrated that the ELISAs were a reliable screening tool for the determination of atrazine in soil matrices. A good correlation of results obtained by ELISAs and gas chromatography within the concentration range of 0-380 mu g kg(-1) was found in the samples collected from two maize fields (r PAb ELISA-GC) = 0.97, n = 21; r (MAb ELISA GC) = 0.94, n = 21]. A somewhat lower correlation was found for the samples collected from ten additional fields within the concentration range of 0-390 mu g kg(-1) [r(PAb ELISA-GC) = 0.91, n = 15; r(MAb ELISA-GC) = 0.87, n = 15)]. The results obtained by PAb and MAb ELISAs showed no significant difference although the sensitivity of the PAb method was by one order of magnitude higher than that based on MAb.
引用
收藏
页码:135 / 144
页数:10
相关论文
共 21 条
[1]   DETERMINATION OF ATRAZINE RESIDUES IN WATER AND SOIL BY ENZYME-IMMUNOASSAY [J].
BUSHWAY, RJ ;
PERKINS, B ;
SAVAGE, SA ;
LEKOUSI, SJ ;
FERGUSON, BS .
BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY, 1988, 40 (05) :647-654
[2]   DETERMINATION OF ATRAZINE RESIDUES IN FOOD BY ENZYME-IMMUNOASSAY [J].
BUSHWAY, RJ ;
PERKINS, B ;
SAVAGE, SA ;
LEKOUSI, SL ;
FERGUSON, BS .
BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY, 1989, 42 (06) :899-904
[3]   COMPARISON OF ENZYME-LINKED-IMMUNOSORBENT-ASSAY AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY FOR THE ANALYSIS OF ATRAZINE IN WATER FROM CZECHOSLOVAKIA [J].
BUSHWAY, RJ ;
PERKINS, LB ;
FUKAL, L ;
HARRISON, RO ;
FERGUSON, BS .
ARCHIVES OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY, 1991, 21 (03) :365-370
[4]  
DANKWARDT A, 1995, ANAL LETT, V28, P621, DOI 10.1080/00032719508001122
[5]   EVALUATION OF ATRAZINE SOIL EXTRACTION METHODS FOR THE DETERMINATION BY ENZYME-IMMUNOASSAY AND GAS-CHROMATOGRAPHY [J].
DELVALLE, PL ;
NELSON, JO .
ARCHIVES OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY, 1994, 27 (03) :375-383
[6]   ENZYME IMMUNOASSAYS FOR S-TRIAZINE HERBICIDES AND THEIR APPLICATION IN ENVIRONMENTAL AND FOOD ANALYSIS [J].
FRANEK, M ;
KOLAR, V ;
EREMIN, SA .
ANALYTICA CHIMICA ACTA, 1995, 311 (03) :349-356
[7]   A NEW MONOCLONAL-ANTIBODY FOR THE SENSITIVE DETECTION OF ATRAZINE WITH IMMUNOASSAY IN MICROTITER PLATE AND DIPSTICK FORMAT [J].
GIERSCH, T .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1993, 41 (06) :1006-1011
[8]   ENZYME-IMMUNOASSAY FOR THE DETERMINATION OF ATRAZINE RESIDUES IN SOIL [J].
GOH, KS ;
HERNANDEZ, J ;
POWELL, SJ ;
GARRETSON, C ;
TROIANO, J ;
RAY, M ;
GREENE, CD .
BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY, 1991, 46 (01) :30-36
[9]   ATRAZINE SOIL RESIDUE ANALYSIS BY ENZYME-IMMUNOASSAY - SOLVENT EFFECT AND EXTRACTION EFFICIENCY [J].
GOH, KS ;
HERNANDEZ, J ;
POWELL, SJ ;
GREENE, CD .
BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY, 1990, 45 (02) :208-214
[10]   Comparison of an enzyme immunoassay with gas chromatography for atrazine determination in water and soil [J].
Jenks, BM ;
Roeth, FW ;
Martin, AR .
BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY, 1997, 58 (05) :696-703