Analysis of 5′-upstream non-coding region of FI-carboxymethyl cellulase gene from Aspergillus aculeatus

被引：4

作者：

Ooi, T

Morotomi, K

Kinoshita, S ^{[1
]}

Honda, J

Ueda, S

Arai, M

机构：

[1] Hokkaido Univ, Grad Sch Engn, Dept Mol Chem, Kita Ku, Sapporo, Hokkaido 0608628, Japan

[2] Utsunomiya Univ, Fac Agr, Dept Bioprod Sci, Utsunomiya, Tochigi 3218505, Japan

[3] Osaka Prefecture Univ, Coll Agr, Fac Appl Biol Chem, Osaka 5938531, Japan

来源：

BIOTECHNOLOGY LETTERS | 1999年 / 21卷 / 09期

关键词：

Aspergillus aculeatus; carbon catabolite repression; cellulase; promoter;

D O I：

10.1023/A:1005534925774

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The 5'-upstream non-coding region of an FI-carboxymethyl cellulase (CMCase) gene of Aspergillus aculeatus No. F-50 was obtained and sequenced up to -777 nucleotide in pCMG23. The 5'-upstream region of different lengths were constructed and fused to a reporter gene (Escherichia coli lacZ) in pAN923-42BD, and the resulted constructs were introduced into the A. nidulans. The beta-galactosidase activities of the transformant with 5'-upstream fragment of larger than 319 bp were expressed by the induction, but that of 109 bp drastically decreased to the basal level. This suggests that the region between -109 and -319 of the 5'-upstream non-coding region is involved in the regulation of the FI-CMCase expression.

引用

页码：735 / 739

页数：5

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[3] 2 DIFFERENT, ADJACENT AND DIVERGENT ZINC-FINGER BINDING-SITES ARE NECESSARY FOR CREA-MEDIATED CARBON CATABOLITE REPRESSION IN THE PROLINE GENE-CLUSTER OF ASPERGILLUS-NIDULANS [J].