Nutrient metabolism in pancreatic islets from protein malnourished rats

被引:14
作者
Sener, A
Reusens, B
Remacle, C
Hoet, JJ
Malaisse, WJ
机构
[1] FREE UNIV BRUSSELS,EXPT MED LAB,ERASMUS SCH MED,B-1070 BRUSSELS,BELGIUM
[2] CATHOLIC UNIV LEUVEN,FAC SCI,CELL BIOL LAB,B-3000 LOUVAIN,BELGIUM
[3] CATHOLIC UNIV LEUVEN,WORLD HLTH ORG,COLLABORATING CTR DEV BIOL ENDOCRINE PANCREAS,B-3000 LOUVAIN,BELGIUM
关键词
D O I
10.1006/bmme.1996.0066
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The metabolism of D-[5-H-3]glucose, D-[3,4-C-14]glucose, [2-H-3]glycerol, L-[U-C-14]glutamine, L-[1-C-14]leucine, and L-[U-C-14]leucine was investigated in pancreatic islets isolated from either control rats or animals fed a low-protein isocaloric diet, containing 8% instead of 20% protein, during both fetal and postnatal life. In the latter animals, decreases in body weight, plasma insulin concentration, and insulinogenic index were associated with two major anomalies of islet nutrient metabolism. First, an imbalance between oxidative and anaerobic glycolysis was found in the islets of rats fed the low-protein isocaloric diet. It coincided with a decreased circulation in the glycerol phosphate shuttle, as judged by the generation of (HOH)-H-3 hom [2-H-3]glycerol, and was probably attributable to the deficiency of mitochondrial FAD-linked glycerophosphate dehydrogenase previously documented in islet homogenates of the rats fed low protein. Second, the transamination of L-leucine to 2-ketoisocaproate was decreased in the low-protein-fed rats, while the oxidative decarboxylation of the 2-keto acid and the further catabolism of isovaleryl CoA occurred at normal rates when expressed relative to the initial transamination rate. These metabolic anomalies may account, in part at least, for the impairment of insulin release in protein malnutrition. (C) 1996 Academic Press, Inc.
引用
收藏
页码:62 / 67
页数:6
相关论文
共 17 条
  • [1] Bergmeyer HU, 1974, METHOD ENZYMAT AN, P1205
  • [2] HEXOSE METABOLISM IN PANCREATIC-ISLETS - PYRUVATE-CARBOXYLASE ACTIVITY
    CURI, R
    CARPINELLI, AR
    MALAISSE, WJ
    [J]. BIOCHIMIE, 1991, 73 (05) : 583 - 586
  • [3] ISLET FUNCTION IN OFFSPRING OF MOTHERS ON LOW-PROTEIN DIET DURING GESTATION
    DAHRI, S
    SNOECK, A
    REUSENSBILLEN, B
    REMACLE, C
    HOET, JJ
    [J]. DIABETES, 1991, 40 : 115 - 120
  • [4] DAHRI S, 1994, DIABETOLOGIA, V37, pA80
  • [5] TYPE-2 (ON-INSULIN-DEPENDENT) DIABETES-MELLITUS - THE THRIFTY PHENOTYPE HYPOTHESIS
    HALES, CN
    BARKER, DJP
    [J]. DIABETOLOGIA, 1992, 35 (07) : 595 - 601
  • [6] METABOLISM OF 4-METHYL-2-OXOPENTANOATE IN RAT PANCREATIC-ISLETS
    HUTTON, JC
    SENER, A
    MALAISSE, WJ
    [J]. BIOCHEMICAL JOURNAL, 1979, 184 (02) : 291 - 301
  • [7] MULTIPLE EFFECTS OF LEUCINE ON GLUCAGON, INSULIN, AND SOMATOSTATIN SECRETION FROM THE PERFUSED RAT PANCREAS
    LECLERCQMEYER, V
    MARCHAND, J
    WOUSSENCOLLE, MC
    GIROIX, MH
    MALAISSE, WJ
    [J]. ENDOCRINOLOGY, 1985, 116 (03) : 1168 - 1174
  • [8] INTERFERENCE OF GLYCOGENOLYSIS WITH GLYCOLYSIS IN PANCREATIC-ISLETS FROM GLUCOSE-INFUSED RATS
    MALAISSE, WJ
    MAGGETTO, C
    LECLERCQMEYER, V
    SENER, A
    [J]. JOURNAL OF CLINICAL INVESTIGATION, 1993, 91 (02) : 432 - 436
  • [9] 3-O-METHYL-D-GLUCOSE TRANSPORT IN TUMORAL INSULIN-PRODUCING CELLS
    MALAISSE, WJ
    GIROIX, MH
    MALAISSELAGAE, F
    SENER, A
    [J]. AMERICAN JOURNAL OF PHYSIOLOGY, 1986, 251 (06): : C841 - C846
  • [10] MALAISSE WJ, 1988, BIOCHIM BIOPHYS ACTA, V971, P246, DOI 10.1016/0167-4889(88)90139-5