Characterization of chromosome changes in two human prostatic carcinoma cell lines (PC-3 and DU145) using chromosome painting and comparative genomic hybridization

被引：23

作者：

Bernardino, J ^{[1
]}

Bourgeois, CA ^{[1
]}

Muleris, M ^{[1
]}

Dutrillaux, AM ^{[1
]}

Malfoy, B ^{[1
]}

Dutrillaux, B ^{[1
]}

机构：

[1] NATL CTR SCI RES,DEPT MOL CYTOGENET,PARIS,FRANCE

来源：

CANCER GENETICS AND CYTOGENETICS | 1997年 / 96卷 / 02期

关键词：

D O I：

10.1016/S0165-4608(96)00258-0

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Using chromosome painting, a study of chromosomal abnormalities has been performed in two prostatic carcinoma cell lines, PC-3 and DU145. In PC-3, this analysis revealed a highly rearranged hypotriploid karyotype with 54 to 61 chromosomes and numerous rearrangements of chromosomes 1, 3, 5, 8, 10, and 14. At passage 73, DU145 had a hypotriploid karyotype with few rearrangements of chromosomes 1, 3, 5, 12, 13, and 20, whereas at passage 153, this cell line showed a near-tetraploid karyotype with a great number of rearrangements involving chromosomes 3, 6, 8, 20, 12, and 17. A single rearrangement was shared by the 2 cell lines, an i(5)(p10). A comparative genomic hybridization study demonstrated a noticeable amplification of bands 10q22.3-q23 and 14q22-q24 in the PC-3 cell line. No amplification signal was detected for DU145. (C) Elsevier Science Inc., 1997.

引用

页码：123 / 128

页数：6

共 34 条

[1] INTERPHASE CYTOGENETICS OF PROSTATIC ADENOCARCINOMA AND PRECURSOR LESIONS - ANALYSIS OF 25 RADICAL PROSTATECTOMIES AND 17 ADJACENT PROSTATIC INTRAEPITHELIAL NEOPLASIAS [J].