Production of minicellulosomes from Clostridium cellulovorans in Bacillus subtilis WB800

被引：62

作者：

Cho, HY

Yukawa, H

Inui, M

Doi, RH ^{[1
]}

Wong, SL

机构：

[1] Univ Calif Davis, Sect Mol & Cellular Biol, Davis, CA 95616 USA

[2] Res Inst Innovat Technol Earth, Kyoto, Japan

[3] Univ Calgary, Dept Biol Sci, Calgary, AB T2N 1N4, Canada

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2004年 / 70卷 / 09期

关键词：

D O I：

10.1128/AEM.70.9.5704-5707.2004

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Two genes encoding EngB endoglucanase and mini-CbpAl scaffolding protein of Clostridium cellulovorans were constructed and coexpressed in Bacillus subtilis WB800. The resulting minicellulosomes were isolated by gel filtration chromatography and characterized. Biochemical and immunological evidence indicated that fraction 11 contained minicellulosomes consisting of mini-CbpAl and EngB. The in vivo synthesis of minicellulosomes suggests that it will be possible in the future to insert into B. subtilis cellulosomal genes that will allow growth on cellulosic materials and the production of various designer cellulosomes with specific functions.

引用

收藏

页码：5704 / 5707

页数：4

相关论文

共 28 条

[1] EXPRESSION OF A CELE GENE FROM CLOSTRIDIUM-THERMOCELLUM IN BACILLUS [J].

AMINOV, RI ;

GOLOVCHENKO, NP ;

OHMIYA, K .

JOURNAL OF FERMENTATION AND BIOENGINEERING, 1995, 79 (06) :530-537

[2] Cellulosome and noncellulosomal cellulases of Clostridium cellulovorans [J].

Doi, RH ;

Park, JS ;

Liu, CC ;

Malburg, LM ;

Tamaru, Y ;

Ichiishi, A ;

Ihrahim, A .

EXTREMOPHILES, 1998, 2 (02) :53-60

[3] Cellulosomes from mesophilic bacteria [J].

Doi, RH ;

Kosugi, A ;

Murashima, K ;

Tamaru, Y ;

Han, SO .

JOURNAL OF BACTERIOLOGY, 2003, 185 (20) :5907-5914

[4]

Doi RH, 2001, CHEM REC, V1, P24

[5] NUCLEOTIDE-SEQUENCE AND CHARACTERISTICS OF ENDOGLUCANASE GENE ENGB FROM CLOSTRIDIUM-CELLULOVORANS [J].

FOONG, F ;

HAMAMOTO, T ;

SHOSEYOV, O ;

DOI, RH .

JOURNAL OF GENERAL MICROBIOLOGY, 1991, 137 :1729-1736

[6] Characterization of xylanolytic enzymes in Clostridium cellulovorans:: Expression of xylanase activity dependent on growth substrates [J].

Kosugi, A ;

Murashima, K ;

Doi, RH .

JOURNAL OF BACTERIOLOGY, 2001, 183 (24) :7037-7043

[7] Properties of exgS, a gene for a major subunit of the Clostridiun cellulovorans cellulosome [J].

Liu, CC ;

Doi, RH .

GENE, 1998, 211 (01) :39-47

[8] Synergistic effects of cellulosomal xylanase and cellulases from Clostridium cellulovorans on plant cell wall degradation [J].

Murashima, K ;

Kosugi, A ;

Doi, RH .

JOURNAL OF BACTERIOLOGY, 2003, 185 (05) :1518-1524

[9] Synergistic effects on crystalline cellulose degradation between cellulosomal cellulases from Clostridium cellulovorans [J].

Murashima, K ;

Kosugi, A ;

Doi, RH .

JOURNAL OF BACTERIOLOGY, 2002, 184 (18) :5088-5095

[10] Heterologous production of Clostridium cellulovorans engB, using protease-deficient Bacillus subtilis, and preparation of active recombinant cellulosomes [J].

Murashima, K ;

Chen, CL ;

Kosugi, A ;

Tamaru, Y ;

Doi, RH ;

Wong, SL .

JOURNAL OF BACTERIOLOGY, 2002, 184 (01) :76-81