Cloning of rat and mouse aquaporin-2 gene promoters and identification of a negative cis-regulatory element

被引:32
作者
Rai, T
Uchida, S
Marumo, F
Sasaki, S
机构
关键词
kidney-specific gene expression; transcriptional factor; water channel;
D O I
10.1152/ajprenal.1997.273.2.F264
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
The promoters of rat and mouse aquaporin-2 (AQP-2) genes were cloned and compared with that of human genes. Nucleotide identity up to -593 bp was 62%, and consensus sequences such as TATA box and adenosine 3',5'-cyclic monophosphate responsive element were conserved. Deoxyribonuclease I footprint assay revealed a footprinted region at -210 to -184 bp in rat AQP-8 gene promoter produced by nuclear extract from nonexpressing (liver) tissue. The sequence of this region included a GATA motif but otherwise showed no homology with any other previously known cis-elements. Electromobility shift assay and ultraviolet crosslinking analysis confirmed that specific binding proteins to this element were present in kidney, spleen, and liver and that these proteins were distinct from GATA factors. Both deletion and mutation of this cis-element abolished the protein DNA binding and increased promoter activity in in vitro reporter gene assay using rat cultured hepatocyte Ac2F cells, suggesting the negative regulatory role of this cis-element. These results indicate that tissue-specific expression of AQP-8 gene may in part be regulated by this novel negative acting cis-element.
引用
收藏
页码:F264 / F273
页数:10
相关论文
共 33 条
[1]  
ADACHI S, 1994, J BIOL CHEM, V269, P17677
[2]   AQUAPORINS - A FAMILY OF WATER CHANNEL PROTEINS [J].
AGRE, P ;
SASAKI, S ;
CHRISPEELS, MJ .
AMERICAN JOURNAL OF PHYSIOLOGY, 1993, 265 (03) :F461-F461
[3]   MOLECULAR-CLONING OF THE RECEPTOR FOR HUMAN ANTIDIURETIC-HORMONE [J].
BIRNBAUMER, M ;
SEIBOLD, A ;
GILBERT, S ;
ISHIDO, M ;
BARBERIS, C ;
ANTARAMIAN, A ;
BRABET, P ;
ROSENTHAL, W .
NATURE, 1992, 357 (6376) :333-335
[4]   A SINGLE POLYPEPTIDE POSSESSES THE BINDING AND TRANSCRIPTION ACTIVITIES OF THE ADENOVIRUS MAJOR LATE TRANSCRIPTION FACTOR [J].
CHODOSH, LA ;
CARTHEW, RW ;
SHARP, PA .
MOLECULAR AND CELLULAR BIOLOGY, 1986, 6 (12) :4723-4733
[5]   REQUIREMENT OF HUMAN RENAL WATER CHANNEL AQUAPORIN-2 FOR VASOPRESSIN-DEPENDENT CONCENTRATION OF URINE [J].
DEEN, PMT ;
VERDIJK, MAJ ;
KNOERS, NVAM ;
WIERINGA, B ;
MONNENS, LAH ;
VANOS, CH ;
VANOOST, BA .
SCIENCE, 1994, 264 (5155) :92-95
[6]   FIREFLY LUCIFERASE GENE - STRUCTURE AND EXPRESSION IN MAMMALIAN-CELLS [J].
DEWET, JR ;
WOOD, KV ;
DELUCA, M ;
HELINSKI, DR ;
SUBRAMANI, S .
MOLECULAR AND CELLULAR BIOLOGY, 1987, 7 (02) :725-737
[7]   ACCURATE TRANSCRIPTION INITIATION BY RNA POLYMERASE-II IN A SOLUBLE EXTRACT FROM ISOLATED MAMMALIAN NUCLEI [J].
DIGNAM, JD ;
LEBOVITZ, RM ;
ROEDER, RG .
NUCLEIC ACIDS RESEARCH, 1983, 11 (05) :1475-1489
[8]   CLONING AND EXPRESSION OF AQP3, A WATER CHANNEL FROM THE MEDULLARY COLLECTING DUCT OF RAT-KIDNEY [J].
ECHEVARRIA, M ;
WINDHAGER, EE ;
TATE, SS ;
FRINDT, G .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (23) :10997-11001
[9]   Repressive regulation of the aquaporin-2 gene [J].
Furuno, M ;
Uchida, S ;
Marumo, F ;
Sasaki, S .
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL FLUID AND ELECTROLYTE PHYSIOLOGY, 1996, 271 (04) :F854-F860
[10]   FUNCTIONAL-CHARACTERIZATION AND CELL IMMUNOLOCALIZATION OF AQP-CD WATER CHANNEL IN KIDNEY COLLECTING DUCT [J].
FUSHIMI, K ;
SASAKI, S ;
YAMAMOTO, T ;
HAYASHI, M ;
FURUKAWA, T ;
UCHIDA, S ;
KUWAHARA, M ;
ISHIBASHI, K ;
KAWASAKI, M ;
KIHARA, I ;
MARUMO, F .
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY, 1994, 267 (04) :F573-F582