Uptake and degradation of radioactively labelled albumin microspheres as markers for Kupffer cell phagocytosis

被引：7

作者：

Kindberg, GM ^{[1
]}

Tolleshaug, H ^{[1
]}

Skotland, T ^{[1
]}

机构：

[1] Nycomed Imaging, Res & Dev, N-0401 Oslo, Norway

来源：

CELL AND TISSUE RESEARCH | 2000年 / 300卷 / 03期

关键词：

Kupffer cells; albumin microspheres; liposomes; phagocytosis; degradation; rat (Wistar);

D O I：

10.1007/s004410000229

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Rats were injected with liposomes containing iodixanol (CTP10 Injection; 100 mg iodine per kg body weight) followed by a second injection of I-125-tyramine-cellobiose-albumin microspheres. The amounts of phagocytosed and degraded labelled albumin in liver were measured. A reduced uptake and degradation of albumin microspheres was observed when the labelled microspheres were injected 2 h or 24 h after the liposomes compared with that obtained in control animals receiving saline. No effect on the uptake and degradation of labelled microspheres was observed when the time lag between the injection of liposomes and labelled microspheres was 1 week. The data show that the uptake and degradation of I-125-tyramine-cellobiose-albumin microspheres can be used as indicators of Kupffer cell phagocytotic function following drug uptake by these cells.

引用

页码：397 / 400

页数：4

共 19 条

[1] INVIVO UPTAKE OF CHYLOMICRON (H-3)-LABELED RETINYL ESTER BY RAT-LIVER - EVIDENCE FOR RETINOL TRANSFER FROM PARENCHYMAL TO NON-PARENCHYMAL CELLS [J].