Immunohistochemical detection of steroid receptors in breast cancer: a working protocol

被引：150

作者：

Leake, R

Barnes, D

Pinder, S

Ellis, I

Anderson, L

Anderson, T

Adamson, R

Rhodes, T

Miller, I

Walker, R

机构：

[1] Univ Glasgow, Dept Biochem & Mol Biol, Glasgow G12 8QQ, Lanark, Scotland

[2] Guys Hosp, Imperial Canc Res Fund, Breast Pathol Lab, London SE1 9RT, England

[3] City Hosp Nottingham, Dept Histopathol, Nottingham NG5 1PB, England

[4] Christie Hosp NHS Trust, Dept Clin Res, Manchester M20 4BX, Lancs, England

[5] Western Gen Hosp, Dept Pathol, Edinburgh EH4 2XU, Midlothian, Scotland

[6] Crosshouse Hosp, Area Lab, Kilmarnock KA2 0BE, Scotland

[7] UCL, Sch Med, Dept Histopathol, London WC1E 6JJ, England

[8] Leicester Royal Infirm, Dept Pathol, Leicester LE2 7LX, Leics, England

来源：

JOURNAL OF CLINICAL PATHOLOGY | 2000年 / 53卷 / 08期

关键词：

breast cancer; oestrogen receptor; tamoxifen; immunohistochemistry; external quality control;

D O I：

10.1136/jcp.53.8.634

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

The biochemical assay for the oestrogen receptor has shown the clinical value of knowing the concentration of the receptor within tissue. The immunohistochemical assay is rapidly taking over from the biochemical assay. Therefore, it is vital to have an equivalent scoring system that will have the same predictive value. This paper reports both a practical protocol and a scoring system that should achieve this aim. This approach should be applicable to many more biomarkers detected by immunohistochemistry.

引用

页码：634 / 635

页数：2

共 4 条

[1] Increased use of immunohistochemistry for oestrogen receptor measurement in mammary carcinoma: The need for quality assurance [J].