Tamoxifen induced apoptosis in ZR-75 breast cancer xenografts antedates tumour regression

被引:42
作者
Cameron, DA [1 ]
Ritchie, AA [1 ]
Langdon, S [1 ]
Anderson, TJ [1 ]
Miller, WR [1 ]
机构
[1] UNIV EDINBURGH,DEPT PATHOL,EDINBURGH,MIDLOTHIAN,SCOTLAND
关键词
apoptosis; mitosis; necrosis; regression; tamoxifen; ZR-75-1; MDA-MB-231;
D O I
10.1023/A:1005850827825
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
The ZR-75-1 ER positive breast cancer cell line, xenografted in female nude mice, has been used to determine the effect of tamoxifen on cell proliferation (as measured by mitosis) and cell death (as evidenced by apoptosis and necrosis). After 2 days treatment, there was a significant rise in apoptosis (p < 0.05), whereas a fall in mitosis was not apparent until 7 days (p < 0.05). Furthermore there was an increase in the apoptotic : mitotic ratio on day 7 (p < 0.05). These changes antedated tumour regression, which did reach not significance until day 14. Tamoxifen did not increase necrosis (which significantly decreased in treated tumours once they had regressed (p < 0.01)). In contrast tamoxifen treatment of xenografted MDA-MB-231 ER-negative breast cancer cells produced no significant effects on growth, apoptosis, or mitosis. This study presents clear evidence for tamoxifen inducing apoptosis in ZR-75-1 xenografts (but not MDA-MB-231 tumours). Since changes in apoptosis and mitosis antedate tumour regression, their assessment may provide the potential by which to predict tumour response to tamoxifen therapy.
引用
收藏
页码:99 / 107
页数:9
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