A sensitive and robust assay for urokinase and tissue-type plasminogen activators (uPA and tPA) and their inhibitor type I (PAI-1) in breast tumor cytosols

被引：85

作者：

Grebenschikov, N

GeurtsMoespot, A

DeWitte, H

Heuvel, J

Leake, R

Sweep, F

Benraad, T

机构：

[1] UNIV NIJMEGEN, ACAD HOSP ST RADBOUD, DEPT EXPT & CHEM ENDOCRINOL, NL-6500 HB NIJMEGEN, NETHERLANDS

[2] UNIV GLASGOW, DEPT BIOCHEM, GLASGOW G12 8QQ, LANARK, SCOTLAND

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MARKERS | 1997年 / 12卷 / 01期

关键词：

cancer; plasminogen activators; chicken Ab; immunoassay compared;

D O I：

10.1177/172460089701200102

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

uPA and PAI-1 are becoming established as amongst the most effective markers of poor prognosis for patients with node-negative breast cancer; tPA is an index of longer survival. This paper describes a sensitive ELISA for the measurement of uPA, tPA and PAI-1 in breast cancer cytosols. The structure of the assay involves coating Ab (sheep alpha-Chicken, IgY), catching Ab (chicken alpha-analyte), tagging Ab (rabbit alpha-rabbit IgG) labelled with HRP. The assay has a high degree of accuracy and specificity. Comparison with the American Diagnostica kits shows the results' equivalence for PAI-1 and tPA. For uPA results of the assay were twice as high. The assay is sensitive and relatively inexpensive. It is the first published assay to yield strictly comparative values for uPA, tPA and PAI-1 in tissue extracts and is readily subject to external quality control.

引用

页码：6 / 14

页数：9

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