Isolation and partial characterization of pepsin-soluble collagen from the skin of grass carp (Ctenopharyngodon idella)

被引:196
作者
Zhang, Yan
Liu, Wentao
Li, Guoying [1 ]
Shi, Bi
Miao, Yuqing
Wu, Xiaohua
机构
[1] Sichuan Univ, Minist Educ, Key Lab Leather Chem & Engn, Chengdu 610065, Peoples R China
[2] Zhejiang Normal Univ, Coll Chem & Life Sci, Jinhua 321004, Peoples R China
基金
中国国家自然科学基金;
关键词
skin collagen; grass carp; freshwater fish; isolation; characterization;
D O I
10.1016/j.foodchem.2006.09.053
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
Pepsin-soluble collagen was extracted from the skin of grass carp (Ctenopharyngodon idella) with a yield of 46.6%, on a dry weight basis. Electrophoretic patterns showed that the collagen contained alpha 1 and alpha 2 chains, similar to those of calf skin collagen. The imino acid content of the collagen from grass carp skin was much lower than those of mammalian's collagens, as also were the transition temperature and denaturation temperature which were only 24.6 degrees C and 28.4 degrees C respectively. Peptide maps of the collagen digested by trypsin and V8 protease showed different peptide fragments from those of calf skin collagen and other fish skin collagens, suggesting differences in amino acid sequences and collagen conformation. In addition, the lyophilized collagen sponge from grass carp skin had a uniform and regular network structure, just like calf skin collagen sponge. These results suggest that grass carp skin has potential for use as a supplementary source of collagen. (c) 2006 Elsevier Ltd. All rights reserved.
引用
收藏
页码:906 / 912
页数:7
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