Rapid whole protein quantitation of staphylococcal enterotoxins A and B by liquid chromatography/mass spectrometry

被引：31

作者：

Sospedra, Isabel ^{[1
]}

Soler, Carla ^{[1
]}

Manes, Jordi ^{[1
]}

Miguel Soriano, Jose ^{[1
]}

机构：

[1] Univ Valencia, Fac Pharm, Dept Prevent Med & Publ Hlth, E-46100 Burjassot, Spain

来源：

JOURNAL OF CHROMATOGRAPHY A | 2012年 / 1238卷

关键词：

LC-ESI/MS; Staphyloccocus aureus; Staphylococcal enterotoxins; Milk; Fruit juice; MASS-SPECTROMETRY; QUANTIFICATION; IDENTIFICATION; IONIZATION; EXOTOXINS; WARFARE; TIME;

D O I：

10.1016/j.chroma.2012.03.022

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Staphylococcus aureus is an important pathogen and has been indicated as the fifth causative agent of food-borne human illness throughout the world. Staphylococcal enterotoxins (SEs) are toxic compounds excreted mainly by strains of S. aureus. Among these toxins, enterotoxins A (SEA) and B (SEB) are both of the most prevalent compounds in staphylococcal food poisoning. In this work, reverse phase liquid chromatography coupled to ESI mass spectrometry (LC-ESI/MS) has been applied for its rapid identification and quantification. Limit of detection (LOD) values were 0.5 and 0.2 ng for SEA and SEB, respectively and limit of quantification (LOQ) value was 1 ng for both enterotoxins. SEA and SEB have been analyzed as intact proteins in milk and fruit juices. Analytical methods are essential for routine monitoring purposes and safeguard public health and the proposed technique can detect and quantify successfully SEA and SEB in food samples. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：54 / 59

页数：6

共 39 条

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