A validated method for quantitative determination of saponins in notoginseng (Panax notoginseng) using high-performance liquid chromatography with evaporative light-scattering detection

被引：47

作者：

Li, WK

Fitzloff, JF

机构：

[1] Univ Chicago, Coll Pharm, Dept Med Chem & Pharmacog, Chicago, IL USA

[2] Univ Chicago, FFH, Core Analyt Lab, Program Collaborat Res Pharmaceut Sci, Chicago, IL USA

来源：

JOURNAL OF PHARMACY AND PHARMACOLOGY | 2001年 / 53卷 / 12期

关键词：

D O I：

10.1211/0022357011778241

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

A gradient high-performance liquid chromatographic (HPLC) method with evaporative light-scattering detection (ELSD) for the determination of major saponins in a Chinese traditional herbal medicine, Panax notoginseng, is described. Samples were analysed by means of a reverse-phase column (Waters Spherisorb ODS-2, C-18) using acetonitrile and water under gradient conditions as the mobile phase over 60 min. The ELSD used was set at an evaporating temperature of 35degreesC and pressurized air pressure of 3.4 bars. The detection limit (signal/noise > 3) of the saponins was 50 ng. The method was validated by inter- and intra-day assays and recovery tests.

引用

页码：1637 / 1643

页数：7

共 34 条

[1]

[Anonymous], 1996, CHIN J MED CHEM

[2] Rapid determination of artemisinin and related analogues using high-performance liquid chromatography and an evaporative light scattering detector [J].

Avery, BA ;

Venkatesh, KK ;

Avery, MA .

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 1999, 730 (01) :71-80

[3] Effect of Panax notoginseng saponins on sperm motility and progression in vitro [J].

Chen, JC ;

Xu, MX ;

Chen, LD ;

Chen, YN ;

Chiu, TH .

PHYTOMEDICINE, 1998, 5 (04) :289-292

[4] Effect of Panax notoginseng extracts on inferior sperm motility in vitro [J].

Chen, JC ;

Xu, MX ;

Chen, LD ;

Chen, YN ;

Chiu, TH .

AMERICAN JOURNAL OF CHINESE MEDICINE, 1999, 27 (01) :123-128

[5]

CHEN JQ, 1992, ACTA PHARM SINIC, V13, P538

[6]

CHEN JQ, 1994, ACTA PHARM SINIC, V15, P347

[7] Determination of marker constituents in radix Glycyrrhizae and radix Notoginseng by near infrared spectroscopy [J].

Chen, YX ;

Sorensen, LK .

FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY, 2000, 367 (05) :491-496

[8] A COMPARATIVE-STUDY ON COMMERCIAL SAMPLES OF GINSENG-RADIX [J].

CHUANG, WC ;

WU, HK ;

SHEU, SJ ;

CHIOU, SH ;

CHANG, HC ;

CHEN, YP .

PLANTA MEDICA, 1995, 61 (05) :459-465

[9]

Fuzzati N, 2000, J AOAC INT, V83, P820

[10]

GUAN YY, 1994, ACTA PHARM SINIC, V15, P392

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